Planta Med 2011; 77 - PB48
DOI: 10.1055/s-0031-1282302

Responses of zygotic embryos of galbanum in in vitro conditions

N Hadi 1, A Moeini 2, R Omidbaigi 1
  • 1Department of Horticulture, Tarbiat Modares University, Tehran, Iran
  • 2Department of Plant Biotechnology, Tarbiat Modares University, Tehran, Iran

Galbanum (Ferula gummosa Boiss.) is a valuable medicinal-industrial plant native to Iran which is at risk of extinction due to irregular and overharvesting from natural habitats. The objective of the study was to investigate the growth response of zygotic embryos of galbanum, originated from center of Iran, in in vitro conditions. The results however, showed that zygotic embryos of galbanum had not a suitable germination in in vitro conditions. However, the best treatment for in vitro germination of embryos was ¼ MS (MS with ¼ macro elements) medium supplemented with 0.3mg l-1 GA3. The results also showed the embryos had good callus production in ¼ MS medium (MS with ¼ macro elements) supplemented with 2mg l-1 BA and 10mg l-1 NAA.

In this study, the results showed that although applied treatments did not lead to normal germination of zygotic embryos of galbanum in in vitro conditions, but these treatments were able to force zygotic embryos into the callus production phase with good quality and quantity. The pH of primary media for zygotic embryo germination which was adjusted before adding plant growth regulators, can be effective on good callus production. Nevertheless, more experiments are needed to reveal the effect of pH on callogenesis in galbanum plant.

References: 1. Bernard F et al. (2007) Pakistan Journal of Biological Sciences 10: 1977–1983

2. Irvani N et al. (2009) Plant Cell, Tissue and Organ Culture 100: 293–299

3. Tafreshi RS et al. (2008) Iranian Journal of Medicinal Plants 27: 71–81