Tissue culture studies on Semecarpus kathalekanensis an endangered medicinal plant

Semecarpus kathalekanensis Dasappa & M.H.Swaminath is a evergreen tree with very large simple leaves, attains a height of about 30m, belonging to the family Anacardiaceae is a critically endangered swamp tree and consists of major chemical compounds like phenols, biflavanoids and traditionally having high medicinal importance being used as an antimicrobial, antioxidant and anticancer. Hence considering its population, it is an endangered species and endemic to western ghats region of shimoga district in India. The tissue culture of different plant parts was carried out on MS medium using different concentration of plant growth regulators in the culture tubes and the explants were incubated at 25±20 C under 48 hrs photoperiod. Due its high phenolic content callus initiation was not occurred further isolation and identification of endophytic fungi from Semecarpus kathalekanensis plant was performed.

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