Expression of amorpha-4,11-diene synthase (ADS) gene in Iranian Artemisia annua L. genotypes

Malaria disease is caused by unicell of Plasmodium falsiparum. Nowadays, malaria has been reported in more than 100 countries. The artemisinin is a sesquiterpene that is produced by two pathways of isoprenoid and mevalonate in Artemisia annua L. Artemisinin as new and effective drug is widelyused. Artemisia annua is annual plant and native to Asia and most probably China. In this study, six different genotypes Artemisia annua was collected from the province of Golestan. Amorpha-4, 11-diene synthase (ADS) promoter was analyzed by using different cis-elements database of PLATcare, TRANSFAC and PLACE. The result showed that there are different cis-elements responding to plant hormones and abiotic stress in ADS promoter. We also identified two new putative transcription factors in EST library of Artemisia annua then studied the expression of ADS gene and three transcription factors by using real time PCR technique. The result showed that WRKY transcription factor had more important role than other transcription factors.