Planta Med 2011; 77 - PA47
DOI: 10.1055/s-0031-1282243

Total Phenolics of Thymus species from Natural Flora of Antalya

S Elmasulu 1, A Çınar 2, A Bayır 2, IG Deniz 3
  • 1Akdeniz University, Faculty of Agriculture, 07058, Antalya, Turkey
  • 2Bati Akdeniz Agriculture Research Institute, Aksu, 07113, Antalya, Turkey
  • 3Akdeniz University, Faculty of Education, 07058, Antalya, Turkey

The genus Thymus (Lamiaceae) is represented by about 250 taxa (214 species, 36 subspecies) worldwide (1). There are represented by 37 species and 55 taxa (species, subspecies, variety) in Turkey, 27 taxa of which are endemic (2,3,4).

In this study; aerial parts of the flowering 9 perennial Thymus plants from 17 locations were collected from natural flora of Antalya. In order to analyse of the total phenolics in Thymus (leaf and flower) samples, 0.25g of powder material were processed. The ethanol extraction was performed with 25 mL of 70% ethanol at room temperature for 24h using a shaker (5). The total phenolics content was estimated by the Folin-Ciocalteu method (6). Ethanol extractions were santrifuged and 100µl extracts taken, nine hundred microlitres of water were added. 5ml of 1:10 diluted Folin-Ciocalteu reagent and 4ml of sodium carbonate (75g/l) added to the extracts. After 2h of incubation in the dark at room temperature, the absorbance at 765nm was measured.

The total amount of phenolic compounds varied in the range of 45.1–76.4 mgGAE/g at the level of location and 45.1–66.8 mgGAE/g at the level of species. The highest value at the level of location was obtained from Thymus zygioides Griseb., in Saklıkent location. T. sipyleus Boiss. subsp. sipyleus had the highest average at the level of species. T. leucotrichus Halácsy has the lowest average at the level of species.

References: 1. Morales R (2002) The history, botany and taxonomy of the genus Thymus. In: Stahl-Biskup E, Saez F (Eds) Thyme-the Genus Thymus, Taylor-Francis pp.1–43, London.

2. Jalas J (1982) Thymus L. In: Davis, P.H. (Editor), Flora of Turkey and the East Aegean Islands, Vol. 7: 349–382, Edinburgh University Press, Edinburgh.

3. Davis PH, Mill R R and Tan K 1988. Flora of Turkey and the East Aegean Islands (Suppl. I), Vol 10, Edinburgh University Press, Edinburgh.

4.Güner A, Özhatay N, Ekim T and Başer K H C (2000) Flora of Turkey and the East Aegean Islands (Suppl. II), Vol 11, Edinburgh University Press, Edinburgh.

5. Petreska J, Stefova M, Ferreres F, Moreno DA, Tomas-Barberan FA, Stefkov G, Kulevanova S, Gil-Izquierdo A (2011) Food Chem 125: 13–20

6. Spanos G and Wrolstad RE (1990)J Agric Food Chem 38: 1565–1571.