Planta Med 2011; 77 - PA44
DOI: 10.1055/s-0031-1282240

Quantification of scopoletin in Artemisia annua L. using HPLC-ED

L Klepo 1, A Copra Janicijevic 1, E Sofic 2, G Kroyer 3, H Muradic 4
  • 1Faculty of Science, Department of Chemistry, Zmaja od Bosne, 71 000 Sarajevo, Bosna i Hercegovina
  • 2Faculty of Pharmacy, University of Sarajevo, Kosevska 4, 71000 Sarajevo, Bosnia and Hercegovina
  • 3Institute of Chemical Engineering, University of Technology Wienna, Getreidemarkt 9/166 A-1060 Vienna Austria
  • 4Karl Franzens, Univercity, Department of Analytical chemistry, Universitatsplatz 1, 8010 Graz Austria

Artemisia annua L. (Asteraceae) contains various phytochemical compounds such as monoterpenoids, sesquiterpenoids, flavonoids, coumarins, sterols etc. Scopoletin (7-hydroxy-6-methoxycoumarin) is coumarin which can be found in plants of family Asteraceae. In this study, using HPLC-ED system, quantitave analysis of scopoletin was carried out in water extracts of A. annua. The shade dried plant material (10g) was powdered and subjected to extraction with petroleum ether in Soxhlet apparatus. The defatted marc was then extracted with ethanol till complete extraction. 100mg of ethanolic extract was weighed accurately and dissolved in 10 mL of water with vigorous shaking. It was then filtered and the volume made up to 100 mL with water (stock solution). HPLC conditions were following: Mobile phase: methanol-acetonitrile-water-acetic acid (20+10+70+1); electrochemical detector with range 50 nA, potential +0.840 V, filter 0.02Hz; flow rate 1 mL/min; temperature 25°C, column: ODS hypersil. Injection volume was 20µl. Concentrations of standard were: 10ng/20µL, 20ng/20µL and 40ng/20µL. Retention time of scopoletin in standard solution and extracts of A.annua was 11.4 minutes. Content of scopoletin in extracts of two samples of A. annua were 109µg/g and 534µg/g dry plant. The result showed that very low concentration of scopoletin in plant extracts can be indentified and quantified.

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