Planta Med 2011; 77 - PA41
DOI: 10.1055/s-0031-1282237

Exploitation of HPTLC for methodology development: quantification, fingerprinting and partition coefficient determination

V Boka 1, V Amountzias 1, A Argyropoulou 1, N Aligiannis 1, A Skaltsounis 1
  • 1Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, University of Athens, Panepistimiopolis Zografou, 157 71, Athens, Greece

High Performance Thin Layer Chromatography (HPTLC) is a simple, modern analytical densitometric analysis technique [1–5]. In the present study, HPTLC methods were developed, validated and compared to analytical techniques routinely used in the laboratory.

The quantification of oleuropein in differently processed extracts of olive leaves was carried out. A calibration curve was created with oleuropein as standard and their content was determined with HPTLC. Quantification was achieved by UV detection at λ 240nm and excellent linear behaviors over the investigated concentrations were observed. The results were statistically compared with the ones obtained from two well established techniques, namely HPLC and NMR, and proved to be precise and accurate.

Furthermore, HPTLC was used for the documentation of the fingerprinting of Genista halacsyi Heldr. in order to detect and quantify the major compounds. The chromatograms allowed the identification of seven main constituents.

Moreover, the possibility of elaborating HPTLC for the determination of the partition coefficients used in counter-current chromatography was examined. The obtained results were successfully applied for the purification of the target compounds of the aforementioned plant, indicating that the partition coefficients could effectively be determined with HPTLC analysis and not necessarily with HPLC.

HPTLC provided reliable results in all the methods which were developed. It was shown to be sensitive, selective, repeatable, easy to handle, requiring low analysis time and less cost per analysis. Overall, HPTLC could be efficiently employed instead of expensive and time-consuming techniques.

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4. Dhalwal K et al. (2010)J Med Plants Res 4: 1289–1296.

5. Plocharz P et al. (2010)J Chromatogr A 1217: 4868–4872.