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Planta Med 2012; 78(1): 90-101
DOI: 10.1055/s-0031-1280117
Analytical Studies
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Metabolomic Differentiation of Maca (Lepidium meyenii) Accessions Cultivated under Different Conditions Using NMR and Chemometric Analysis

Jianping Zhao1 , Bharathi Avula1 , Michael Chan2 , 3 , Céline Clément4 , Michael Kreuzer4 , Ikhlas A. Khan1 , 2
  • 1National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS, USA
  • 2Department of Pharmacognosy, School of Pharmacy, University of Mississippi, University, MS, USA
  • 3British Columbia Institute of Technology, Burnaby, B. C., Canada
  • 4ETH Zurich, Institute of Agricultural Sciences, Zurich, Switzerland
Further Information

Publication History

received January 14, 2011 revised May 25, 2011

accepted July 6, 2011

Publication Date:
19 August 2011 (online)

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Abstract

To gain insights on the effects of color type, cultivation history, and growing site on the composition alterations of maca (Lepidium meyenii Walpers) hypocotyls, NMR profiling combined with chemometric analysis was applied to investigate the metabolite variability in different maca accessions. Maca hypocotyls with different colors (yellow, pink, violet, and lead-colored) cultivated at different geographic sites and different areas were examined for differences in metabolite expression. Differentiations of the maca accessions grown under the different cultivation conditions were determined by principle component analyses (PCAs) which were performed on the datasets derived from their 1H NMR spectra. A total of 16 metabolites were identified by NMR analysis, and the changes in metabolite levels in relation to the color types and growing conditions of maca hypocotyls were evaluated using univariate statistical analysis. In addition, the changes of the correlation pattern among the metabolites identified in the maca accessions planted at the two different sites were examined. The results from both multivariate and univariate analysis indicated that the planting site was the major determining factor with regards to metabolite variations in maca hypocotyls, while the color of maca accession seems to be of minor importance in this respect.

Key words

maca - Lepidium meyenii - Brassicaceae - metabolite - NMR - chemometrics

Supporting Information

References

  • 1 Valentova K, Stejskal D, Bartek J, Dvorackova S, Kren V, Ulrichova J, Simanek V. Maca (Lepidium meyenii) and yacon (Smallanthus sonchifolius) in combination with silymarin as food supplements: in vivo safety assessment.  Food Chem Toxicol. 2008;  46 1006-1013
    PubMedGoogle Scholar
  • 2 Wang Y, Wang Y, McNeil B, Harvey L M. Maca: an Andean crop with multi-pharmacological functions.  Food Res Int. 2007;  40 783-792
    CrossrefPubMedGoogle Scholar
  • 3 Brinckmann J, Smith E. Maca culture of the Junin Plateau.  J Alternat Complement Med. 2004;  10 426-430
    CrossrefPubMedGoogle Scholar
  • 4 Gonzales G F, Gonzales C, Gonzales-Castaneda C. Lepidium meyenii (Maca): a plant from the highlands of Peru–from tradition to science.  Forsch Komplementmed. 2009;  16 373-380
    CrossrefPubMedGoogle Scholar
  • 5 Quiros C F, Cardenas R A. Maca (Lepidium meyenii Walp.). In: Hermann M, Heller J, editors Andean roots and tubers: Ahipa, Arracacha, Maca and Yacon. Rome: International Plant Genetic Resources Institute; 1997: 173-198
    Google Scholar
  • 6 Muhammad I, Zhao J, Khan I A. Maca. In: Coates P, Blackman M, editors Encyclopedia of dietary supplements. 2nd edition New York: Informa Healthcare; 2010: 518-526
    Google Scholar
  • 7 Clement C, Diaz D, Manrique I, Avula B, Khan I A, Ponce Aguirre D D, Kunz C, Mayer A C, Kreuzer M. Secondary metabolites in Maca as affected by hypocotyl color, cultivation history, and site.  Agron J. 2011;  102 431-439
    PubMedGoogle Scholar
  • 8 Humala-Tasso K, Combelles P O. The Maca (Lepidium Meyenii Walpers) general characteristics. In: Humala-Tasso K, Combelles P O, editors The Maca (Lepidium Meyenii Walpers). Paris, France: Inst. andin d'etudes ethnobiologiques; 2007: 22-44
    Google Scholar
  • 9 Gonzales G F, Nieto J, Rubio J, Gasco M. Effect of black maca (Lepidium meyenii) on one spermatogenic cycle in rats.  Andrologia. 2006;  38 166-172
    CrossrefPubMedGoogle Scholar
  • 10 Gonzales G F, Miranda S, Nieto J, Fernandez G, Yucra S, Rubio J, Yi P, Gasco M. Red maca (Lepidium meyenii) reduced prostate size in rats.  Reprod Biol Endocrinol. 2005;  3 5
    CrossrefPubMedGoogle Scholar
  • 11 Clement C, Diaz Grados D A, Avula B, Khan I A, Mayer A C, Ponce Aguirre D D, Manrique I, Kreuzer M. Influence of colour type and previous cultivation on secondary metabolites in hypocotyls and leaves of maca (Lepidium meyenii Walpers).  J Sci Food Agric. 2010;  90 861-869
    CrossrefPubMedGoogle Scholar
  • 12 Ganzera M, Zhao J, Muhammad I, Khan I A. Chemical profiling and standardization of Lepidium meyenii (Maca) by reversed phase high performance liquid chromatography.  Chem Pharm Bull (Tokyo). 2002;  50 988-991
    CrossrefPubMedGoogle Scholar
  • 13 McCollom M M, Villinski J R, McPhail K L, Craker L E, Gafner S. Analysis of macamides in samples of Maca (Lepidium meyenii) by HPLC-UV-MS/MS.  Phytochem Anal. 2005;  16 463-469
    CrossrefPubMedGoogle Scholar
  • 14 van der Kooy F, Maltese F, Choi Y H, Kim H K, Verpoorte R. Quality control of herbal material and phytopharmaceuticals with MS and NMR based metabolic fingerprinting.  Planta Med. 2009;  75 763-775
    Article in Thieme ConnectPubMedGoogle Scholar
  • 15 Ward J L, Baker J M, Beale M H. Recent applications of NMR spectroscopy in plant metabolomics.  FEBS J. 2007;  274 1126-1131
    CrossrefPubMedGoogle Scholar
  • 16 Dini A, Migliuolo G, Rastrelli L, Saturnino P, Schettino O. Chemical composition of Lepidium meyenii.  Food Chem. 1994;  49 347-349
    CrossrefPubMedGoogle Scholar
  • 17 Zhao J, Muhammad I, Dunbar D C, Mustafa J, Khan I A. New alkamides from maca (Lepidium meyenii).  J Agric Food Chem. 2005;  53 690-693
    CrossrefPubMedGoogle Scholar
  • 18 Muhammad I, Zhao J, Dunbar D C, Khan I A. Constituents of Lepidium meyenii ‘maca .  Phytochemistry. 2002;  59 105-110
    CrossrefPubMedGoogle Scholar
  • 19 Mannina L, Cristinzio M, Sobolev A P, Ragni P, Segre A. High-field nuclear magnetic resonance (NMR) study of truffles (Tuber aestivum vittadini).  J Agric Food Chem. 2004;  52 7988-7996
    CrossrefPubMedGoogle Scholar
  • 20 Piacente S, Carbone V, Plaza A, Zampelli A, Pizza C. Investigation of the tuber constituents of maca (Lepidium meyenii Walp.).  J Agric Food Chem. 2002;  50 5621-5625
    CrossrefPubMedGoogle Scholar
  • 21 Mounet F, Moing A, Garcia V, Petit J, Maucourt M, Deborde C, Bernillon S, Le Gall G, Colquhoun I, Defernez M, Giraudel J L, Rolin D, Rothan C, Lemaire-Chamley M. Gene and metabolite regulatory network analysis of early developing fruit tissues highlights new candidate genes for the control of tomato fruit composition and development.  Plant Physiol. 2009;  149 1505-1528
    CrossrefPubMedGoogle Scholar

Prof. Dr. Ikhlas A. Khan

National Center for Natural Products Research
Research Institute of Pharmaceutical Sciences
University of Mississippi

University, MS 38677

USA

Phone: +1 66 29 15 78 21

Fax: +1 66 29 15 79 89

Email: ikhan@olemiss.edu