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Neuropediatrics 2011; 42(02): 67-70
DOI: 10.1055/s-0031-1279785
Original Article
Georg Thieme Verlag Stuttgart · Newyork

The Plasminogen Activator Inhibitor-1 Gene Polymorphism in Determining the Risk of Pediatric Ischemic Stroke − Case Control and Family-Based Study

A. Balcerzyk
1   Department of Biochemistry and Medical Genetics, School of Health Care, Medical University of Silesia, Katowice, Poland
,
I. Żak
1   Department of Biochemistry and Medical Genetics, School of Health Care, Medical University of Silesia, Katowice, Poland
,
E. Emich-Widera
2   Department of Neuropediatrics, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland
,
I. Kopyta
2   Department of Neuropediatrics, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland
,
T. Iwanicki
1   Department of Biochemistry and Medical Genetics, School of Health Care, Medical University of Silesia, Katowice, Poland
,
E. Pilarska
3   Department of Developmental Neurology, Medical University of Gdansk, Gdansk, Poland
,
K. Pienczk-Ręcławowicz
3   Department of Developmental Neurology, Medical University of Gdansk, Gdansk, Poland
,
M. Kaciński
4   Department of Pediatric and Adolescent Neurology, Jagiellonian University Medical College, Kraków, Poland
,
J. Wendorff
5   Department of Neurology, Polish Mother’s Memorial Hospital − Research Institute, Łódź, Poland
,
K. Połatyńska
5   Department of Neurology, Polish Mother’s Memorial Hospital − Research Institute, Łódź, Poland
› Author Affiliations
Further Information

Publication History

Received: 03 February 2011

Accepted: 15 May 2011

Publication Date:
06 June 2011 (online)

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Abstract

Pediatric ischemic stroke, though relatively rare, remains an important medical problem since 20−40% of patients have recurrent strokes and 50−85% of them suffer from long-term neurological deficits. Approximately 20−50% of the affected children have prothrombotic disorders, therefore upon looking for possible genetic causes of the disease we focused on the plasminogen activator inhibitor (PAI-1) − the major inhibitor of fibrinolysis. The aim of the present study was to investigate a possible association between the -675_-674insG PAI-1 gene polymorphism and pediatric ischemic stroke. The study population consisted of 343 individuals: 70 children with ischemic stroke, 140 their biological parents and 133 control children. The PAI-1 gene polymorphism was genotyped using the restriction fragment length polymorphism and was visualized by AgNO3 staining. The transmission/disequilibrium test showed exactly the same transmission of alleles from parents to the affected children (37:37). The case-control model also did not reveal any statistical significance in alleles and genotypes distribution between patients and control children. The obtained results suggest that the 4 G/5 G polymorphism of the PAI-I gene is not a risk factor of ischemic stroke in Polish children.

Key words

ischemic stroke - children - gene polymorphism - plasminogen activator inhibitor-1
 

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