Quantitative Determination of Triperpene Saponins and Alkenated-Phenolics from Labisia pumila Using an LC-UV/ELSD Method and Confirmation by LC-ESI-TOF

Abstract

This study describes the first analytical method for the determination of four triterpene saponins (ardisicrenoside B, ardisiacrispin A, 3-O-α-L-rhamnopyranosyl-(1 → 2)-β-D-glucopyranosyl-(1 → 4)-α-L-arabinopynanosyl cyclamiretin A and ardisimamilloside H) and three alkenated-phenolics (irisresorcinol, belamcandol B, and demethylbelamcandaquinone B) from the leaves, leaves/stems, and roots of Labisia pumila using an HPLC-UV-ELSD method. The separation was achieved using a reversed-phase (C-18) column, PDA and ELS detection, and a water/acetonitrile gradient as the mobile phase. The major triterpenoid (ardisiacrispin A) and irisresorcinol compounds were detected at a concentration as low as 10.0 and 0.2 µg/mL, respectively. Analysis of various samples showed considerable variation of 0.11–2.46 % for the major triterpenoid compound, ardisiacrispin A. LC-mass spectrometry method coupled with electrospray ionization (ESI) is described for the identification of compounds in plant samples. This method involved the use of the [M + Na]⁺ and [M + NH₄]⁺ ions for compounds 1–4 in the positive ion mode with extractive ion chromatogram (EIC).

Key words

Labisia pumila - Myrsinaceae - LC-UV-ELSD - LC-MS

References

• 1 Ezumi M F W, Amrah S S, Suhaimi A W M, Mohsin S S J. Evaluation of the female reproductive toxicity of aqueous extract of Labisia pumila var. alata in rats.  Indian J Pharmacol. 2007;  39 30-32
• 2 Effendy A W M, Siti-Nurtahirah J, Hussin Z M, Zamri-Saad R. The side effects of Kacip Fatimah extract on liver and kidney of white rats.  J Sustain Sci Manage. 2006;  1 40-46
• 3 Norhaiza M, Maziah M, Hakiman M. Antioxidative properties of leaf extracts of a popular Malaysian herb, Labisia pumila.  J Med Plant Res. 2009;  3 217-223
• 4 Stone B C. Notes on the genus Labisia Lindl. (Myrsinaceae).  Malayan Nat J. 1998;  42 43-51
• 5 Mannerås M F, Wan Nazaimoon W M, Lönn M, Gu H F, Östenson C G, Stener-Victorin E. Beneficial metabolic effects of the Malaysian herb Labisia pumila var. alata in a rat model of polycystic ovary syndrome.  J Ethnopharmacol. 2010;  127 346-351
• 6 Jaganath I B, Ng L T. Herbs: the green pharmacy of Malaysia. Kuala Lumpur: Vinpress Sdn. Bhd in collaboration with the Malaysian Agricultural Research and Development Institute (MARDI); 2000: 53-54
• 7 Zakaria M, Mohammed M A. Traditional Malay medicinal plants. Kuala Lumpur: Fajar Bakti; 1994
• 8 Mansor F, Wan Nazaimoon W M, Gu H F, Ostenson C G. Labisia pumila extract regulates body weight and adipokines in ovariectomized rats.  Maturitas. 2009;  62 91-97
• 9 Ali Z, Khan I A. Chemistry of Kacip Fatimah (Labisia pumila).  Phytochemistry. in press
• 10 ICH .Validation of analytical procedures: text and methodology. ICH Harmonized Tripartite Guidelines, November 2005. Available at. http://www.ich.org Accessed December 20, 2010
• 11 Jansakul C, Baumann H, Kenne L, Samuelsson G. Ardisiacrispin A and B, two utero-contracting saponins from Ardisia crispa.  Planta Med. 1987;  53 405-409