Abstract
Kisspeptin (KP)-Kiss1r, a ligand-receptor pair, has recently been implicated as a
pivotal regulator of the neuroendocrine reproductive axis. Kiss1 (encoding KP) as well as Kiss1r (encoding receptor for KP) are expressed in several peripheral tissues including
the pancreas. But the specific role of KP in the physiology of pancreas is still incompletely
deciphered. This study was designed to examine the effect of peripheral KP administration
on basal and glucose-induced plasma insulin (an important pancreatic hormone) secretion
under fed and fasting conditions in the adult male rhesus monkey. A set of 4 chair-restraint
habituated intact adult male rhesus monkeys were assigned to receive intravenous bolus
administration of human kisspeptin-10 (KP10, 50 μg), and vehicle (1 ml) in normal
fed and fasting conditions without or with glucose infusions. Plasma concentrations
of insulin were measured by using a specific IRMA. Glucose infusion significantly
stimulated plasma insulin levels (p<0.005). Vehicle administration did not affect
both basal and glucose stimulated insulin in fed as well as in fasting condition.
KP10 administration had no effect on the basal insulin levels in both fed and fasting
as compared to pretreatment or vehicle treatment levels, while it significantly heightened
glucose stimulated insulin levels (p<0.05) in both fed and fasted monkeys. The present
results show that KP administration does not affect the basal secretion of insulin
under both fed and fasting condition while potentiated the glucose-induced insulin
levels in the adult male rhesus monkey. Therefore, these findings suggest a potential
role of KP in the physiology of pancreas.
Key words
fasting - kisspeptin - kiss1r - insulin - monkey
References
Correspondence
Shahab@qau.edu.pk
