Planta Med 2010; 76 - P643
DOI: 10.1055/s-0030-1264941

Toxicological studies on the Eugenia dysenterica DC and Caryocar brasiliense Cambess leaves in rats

F Elias 1, T Zils 1, FGL Aguiar 1, ELE Barros 1, ME Molica 2, RF Melo 3
  • 1UPIS – Brasília, Fazenda Lagoa Bonita, Planaltina, 700000 Brasília, Brazil
  • 2(1) UPIS – Brasília, Agronomia, Instituto Federal de Brasília, 70000 Planaltina, Brazil
  • 3UPIS – Brasília, Veterinary Medicine, Fazenda Lagoa Bonita, Planaltina, 70000 Brasília, Brazil

Powder extract of E. dysenterica and C. brasiliense leaves proved to be active against Haemonchus contortus, in vivo, in sheep, according to previous work performed by our group. Therefore, toxicology studies become necessary. To this aim, twenty male rats received a powder extract of E. dysenterica and C. brasiliense leaves for 15 days. The leaves were dried at 37°C, ground, mixed and added to animal feed at concentration of 10%, 20% and 30%. A control group was fed only with diet without the mixture of leaves. After 15 days, only rats that received the feed with 30% of powder leaves showed significant weight loss, probably due to nutritional deficit since no feed reduction consumption was observed. A reduction on thymus weight, a discrete degeneration of liver and decrease on creatinin levels were observed on rats that received the major dose after 15 days. No important variation on hemoglobin, hematocrit, leukocytes and total protein was observed. According to these data we can suggest some toxicity when we use a mixture of 30% of powder leaves added to rat feed, and although a reduction of 81% in fecal egg counts per gram has been observed when we used the dose of only 1,2g/kg in sheep, we intent to perform new experiments to analyze the treatment during a extend time period.