Lipolysis effects of lancemaside A and aglycon from Codonopsis lanceolata water extract in primary cultured adipocytes from obese rat fed high calorie high fat diet

We examined the effects of Lancemaside A and aglycon, its acid hydrolysis product from Lancemaside A, isolated from Codonopsis lanceolata water extract on triglyceride and free fatty acid in primary cultured adipocytes from obese rats fed high calorie high fat diet. Animals (6-week old male Sprague-Dawley rats) were administered a 60% high-fat diet for 6 weeks and adipocyte isolated from apididymal fat [1]. Adipocytes cultured with samples for 4 day. Lipids were extracted by Folch method [2]. Lancemaside A and aglycon significantly reduced in lipid droplets, TG and FFA in a concentration-dependent manner. These data suggest that Lancemaside A and aglycon were active compounds of Codonopsis lanceolata, and these samples improves lipid dysregulation. It could be widely utilized in the therapeutic and functional food for obesity patients.

References: 1. Rodbell M. (1964)J. Biol. Chem. 239: 375–380.

2. Folch, J. (1957)J. Biol. Chem. 26:497–509.