Planta Med 2010; 76 - P581
DOI: 10.1055/s-0030-1264879

Hepatoprotective effect of Mahonia oiwakensis stems against carbon tetrachloride hepatotoxicity

C Chen 1, J Chao 1, W Peng 2, M Lee 2, M Lee 3, H Chen 4, H Cheng 5, T Chou 6
  • 1National Defense Medical Center, Graduate Institute of Lift Sciences, 161 Minchuan East Road, Sec. 6, Taipei, Taiwan, 114, R.O.C., 114 Taipei, Taiwan
  • 2China Medical University, Graduate Institute of Chinese Pharmaceutical Science, No.91 Hsueh-Shih Road, Taichung, Taiwan 40402, R.O.C, 40402 Taichung, Taiwan
  • 3Tungs Taichung MetroHarbor Hospital, Department of Medical Research, No.699, Sec.1, Chungchi Rd., Wuchi Township, Taichung County 435, Taiwan, R.O.C, 435 Taichung, Taiwan
  • 4Mingchi University of Technology, Department of Safety, Health and Environmental Engineering, 84 Gungjuan Rd., Taishan, Taipei 24301, Taiwan, R.O.C., 24301 Taipei, Taiwan
  • 5Chung Jen College of Nursing, Health Sciences and Management, No.1–10, Hubei Village, Dalin Township, Chiayi County 622, Taiwan, ROC, 622 Chiayi, Taiwan
  • 6National Defense Medical Center, Graduate Institute of Physiology, 161 Minchuan East Road, Sec. 6, Taipei, Taiwan, 114, R.O.C., 114 Taipei, Taiwan

This study using in vitro and in vivo models investigates hepatoprotective activities of the ethanol extract of Mahonia oiwakensis stems (MOSEtOH). Anti-oxidative activity of MOSEtOH was evaluated by 2, 2-diphenlyl-1-picrylhydrazyl (DPPH) radical. Wistar rats were orally pretreated with MOSEtOH (20, 100 and 500mg/kg) and silymarin (200mg/kg) for three consecutive days with administration of carbon tetrachloride (CCl4) (1ml/kg, 50% CCl4 in olive oil). The results showed that MOSEtOH exhibited anti-oxidative activity in the DPPH (IC50, 0.743mg/mL) assay. Treatment with MOSEtOH (100 and 500mg/kg) or silymarin decreased the AST and ALT levels in serum when compared with CCl4-treated group. Histological analyses also show that MOSEtOH and silymarin reduced the incidence of liver lesions including vacuole formation, neutrophil infiltration and necrosis of hepatocytes induced by CCl4 in rats. Additionally, MOSEtOH and silymarin attenuated the decreased protein activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GRd) and increased malondialdehyde (MDA) and nitric oxide (NO) contents in liver as compared with CCl4-treated group. In conclusion, the MOSEtOH (100 and 500mg/kg) has a strong hepatoprotective effect on CCl4-induced hepatic injury in rats and can be used as pharmacological agent for the prevention of liver disorders.

Fig.1