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DOI: 10.1055/s-0030-1264852
Quantitative analysis of isoflavones in 11 Trifolium spec. from Turkish flora and in some commercially available Trifolium products
The genus Trifolium is represented by 96 species in Turkish Flora [1]. Among these species Trifolium pratense L. is marketed for use in alleviating menopausal symptoms. T. pratense contains the isoflavones, daidzein, formononetin, biochanin A, and genistein. It has been shown that the isoflavonoids lower the cholesterol level, play a role in prevention and medication of some cancer types and help to reduce menopausal symptoms [2–5]. The purpose of this study was to quantify four isoflavones in the 11 Trifolium spec. collected from nature and in some herbal products to determine which sample contains the highest isoflavone amount. A rapid HPLC-DAD method used for determination of isoflavonoids [6]. The method was validated according to the guidelines. T. canescens and T. triocephalum were found as the samples contain higher isoflavonoid amount than T. pratense [Fig.1].
References: 1. Zohary, M. 1970. Trifolium L. In P.H. Davis (Ed) Flora of Turkey and the East Aegan Islands, Edinburg University Press. Vol.3, p.412–413.
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