Fermentation cultures of Candida albicans (strain DSMZ 4884), C. parapsilosis (strain
DSMZ 5197), Penicillium brevicompactum (strain DSMZ 3961), P. chrysogenum (strain
DSMZ 5753), P. glabrum (strain DSMZ 5752) and P. roquefortii (strain DSMZ 5504) are
prepared using various purification steps and cell mill treatment as well as sterilization
and lyophilisation procedures to produce water-soluble substances [1–6]. Homeopathic
(isopathic) dilutions of these starting materials are used as drug products in various
European countries under the trade names of Albicansan, Pefrakehl, Stolonikehl, Notakehl,
Quentakehl, Fortakehl and Exmykehl. The identification of the drug substances are
performed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and capillary electrophoresis
(CE) producing characteristic fingerprints. The SDS-PAGE analysis shows substance-specific
bands with the following relative molecule masses (Mr). The signals obtained by CE
and detection at 260nm show fingerprint electropherograms that are specific for every
substance preparation, making it possible to identify each individual cultured strain.
Molecule Masses from extracts of Candida and Peniciliium species
Table 1
|
Candida sp.
|
Mr [kDa]
|
|
Penicillium sp.
|
>100
|
100–90
|
90–80
|
80–70
|
70–60
|
60–50
|
50–40
|
40–30
|
30–20
|
<20
|
|
C. albicans
|
|
|
85
|
77
|
|
|
|
37/34
|
26
|
16
|
|
C. parapsilosis
|
118
|
|
|
78
|
|
|
41
|
37
|
28
|
|
|
P. brevicompactum
|
|
|
|
|
|
59
|
|
34
|
27/25
|
|
|
P. chrysogenum
|
|
|
|
76
|
|
|
|
|
24
|
16
|
|
P. glabrum
|
|
96
|
|
|
|
57
|
41
|
|
24
|
|
|
P. roquefortii
|
|
|
|
|
67
|
|
50
|
38
|
24
|
|
References: 1. German Homeopathic Pharmacopoeia (HAB) draft monograph (2009) „Candida albicans
e volumine cellulae (lyophil., steril.)“.
2. German Homeopathic Pharmacopoeia (HAB) draft monograph (2009) „Candida parapsilosis
e volumine cellulae (lyophil., steril.)“.
3. German Homeopathic Pharmacopoeia (HAB) draft monograph (2010) „Penicillium brevicompactum
e volumine cellulae (lyophil., steril.)“.
4. German Homeopathic Pharmacopoeia (HAB) draft monograph (2009) „Penicillium chrysogenum
e volumine cellulae (lyophil., steril.)“.
5. German Homeopathic Pharmacopoeia (HAB) draft monograph (2009) „Penicillium glabrum
e volumine cellulae (lyophil., steril.)“.
6. German Homeopathic Pharmacopoeia (HAB) draft monograph (2009) „Penicillium roquefortii
e volumine cellulae (lyophil., steril.)“.
