Development of HPTLC method for quantification of flavonoids in extracts of the selected Potentilla species

Potentilla species (Rosaceae) and their extracts have been highly valued in many different ethnic cultures for hundreds of years throughout the world. Extracts from P. species were and are still applied for the treatment of inflammations, wounds, infections due to bacteria, virus or fungi, diarrhoea, diabetes mellitus and several more ailments. Most of the biological activities of P. extracts can be explained with the high content of polyphenolics e.g. tannins (proanthocyanidins and hydrolysable tannins), numerous flavonoids, coumarins, polyprenols, phenolic carboxylic acids as well as triterpenoids [1]. Our previous study for the simultaneous determination of polyphenolics from P. species has been developed and validated using HPTLC precoated silica gel 60F254 plates with toluen\ethyl formate\formic acid (6:4:1, v/v/v) [2]. The aim of the present study was to determinate of three flavonoids: isoquercitrin (IQ), quercetin 3-glucuronide (QG) and rutin (RT) in the ethyl acetate extracts obtained from aerial parts of the selected Potentilla species: P. nepalensis and Drymocallis rupestris (P. rupestris) by using HPTLC-densitometry method. Chromatography was performed in CAMAG ADC2 (Automatic Development Chamber). HPTLC-DIOL silica gel F254 plates were applied. As a mobile phase ethyl acetate/methyl ethyl ketone/diisopropylether/formic acid (3:10:4:1, v/v/v/v) was used (distance of 7.5cm). Densitometry was carried out by using of Shimadzu CS-9301PC densitometer. The absorption spectra were recorded at 350nm. The proposed HPTLC method was found to be simple, precise and accurate for the quantification of these compounds in plant materials.

Acknowledgements: This study is financially supported by the Polish Ministry of Science and Higher Education (Grant No. N N405 621638)

References: 1. Tomczyk, M., Latté, KP. (2009)J Ethnopharmacol 122:184–204.

2. Tomczyk, M. et al. (2010) Phytochem Anal 21:174–179.