Antitubercular resorcinol analogs and benzenoid C-glucoside from the roots of Ardisia cornudentata
Ardisia cornudentata Mez (Myrsinaceae) is a small shrub, endemic to Taiwan, and distributed at low altitudes in the North and South of the island . The whole plant of this species has been used as folk medicine to eliminate blood stasis, disperse swelling, improve blood circulation, and also as an analgesic . The methanolic extract of the root of this species showed antitubercular activity against Mycobacterium tuberculosis H37RV in vitro. The extract was partitioned into n-hexane, EtOAc, and H2O-soluble layers. The EtOAc-soluble layer showed potent antitubercular activity against M. tuberculosis H37RV in vitro. Bioassay-guided fractionation of the active EtOAc-soluble layer of the roots of A. cornudentata led to the isolation of three new compounds, 3-methoxy-2-methyl-5-pentylphenol (1), 3-methoxy-2-methyl-5-(1′-ketopentyl)phenol (2), and cornudoside (3), together with twenty-six known compounds. Their structures were elucidated by analysis of spectroscopic data. Thirteen of these isolates, 1, 2, 4-6, 9-15 and 21 showed antitubercular activities against M. tuberculosis H37RV in vitro, with MIC values of 2.5–60µg/mL.
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2. Committee on Chinese Medicine and Pharmacy (2003) The Catalogue of Medicinal Plant Resources in Taiwan, Committee on Chinese Medicine and Pharmacy, Department of Health Executive Yuan, Taipei.