Planta Med 2010; 76 - P414
DOI: 10.1055/s-0030-1264712

Alkaloids and phytosterols from Cakile maritima present in UAE

A Al-Azzawi 1, A Al-Guboori 2, A Abdul-Sada 3, M Al-Azzawi 4
  • 1Ras Al Khaimah Medical and Health Sciences University, College Of Pharmaceutical Sciences, Ras Al Khaimah, Pharmaceutical Chemistry, Ras Al Khaimah, Al-Mamoura, Julan POBOX 11172, 11172 Ras Al Khaimah, United Arab Emirates
  • 2Ras Al Khaimah Medical &Health Sciences University, General Education, Ras Al Khaimah, UAE, 11172 Ras Al Khaimah, United Arab Emirates
  • 3University Of Sussex School of Life Science, Department Chemistry and Biochemistry Mass Spectrometry Center, Brighton, BN1 9QJ Brighton, United Kingdom
  • 4University Of Sussex School of Life Science, Department Biology, John Maynard Smith Building, BN1 9QG Brighton, United Kingdom

Cakile maritima is a halophyte exhibiting potential for secondary metabolite production. Plants grown in the United Arab Emirates (UAE) were investigated for the first time for its phytochemical constituents and antibacterial activity. Dried leaves and stems collected from the gardens in Ras Al-Khiama, UAE were (soxhlet) extracted using equal amounts of ethanol and water for three hours. Alkaloids were detected by thin layer chromatography with dichloromethane:ethanol:ammonia as the mobile phase and Dragendorff's spraying reagent. Isolation and purification of alkaloids and phytosterols was performed by column chromatography using dichloromethane and ethanol as the mobile phase in gradient elution technique. The detection of alkaloids and phytosterols was confirmed by gas chromatography-mass spectrometry (GC-MS) analysis and Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS). The bacteriological assays for different plant extracts (aqueous, ethanolic and dichloromethane) were conducted to evaluate the antibacterial effect against gram negative and gram positive bacteria.

Acknowledgements: Dean College of Pharmaceutical Sciences Ras Al Khaimah, Ras Al Khaimah Medical &Health Sciences University, Ras Al Khaimah, UAE. Microbiology department, Ras Al Khaimah Medical &Health Sciences University, Ras Al Khaimah, UAE. Professor Tim flower University Of Sussex School of Life Science Department Biology, John Maynard Smith Building, UK