Alkaloids and phytosterols from Cakile maritima present in UAE
Cakile maritima is a halophyte exhibiting potential for secondary metabolite production. Plants grown in the United Arab Emirates (UAE) were investigated for the first time for its phytochemical constituents and antibacterial activity. Dried leaves and stems collected from the gardens in Ras Al-Khiama, UAE were (soxhlet) extracted using equal amounts of ethanol and water for three hours. Alkaloids were detected by thin layer chromatography with dichloromethane:ethanol:ammonia as the mobile phase and Dragendorff's spraying reagent. Isolation and purification of alkaloids and phytosterols was performed by column chromatography using dichloromethane and ethanol as the mobile phase in gradient elution technique. The detection of alkaloids and phytosterols was confirmed by gas chromatography-mass spectrometry (GC-MS) analysis and Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS). The bacteriological assays for different plant extracts (aqueous, ethanolic and dichloromethane) were conducted to evaluate the antibacterial effect against gram negative and gram positive bacteria.
Acknowledgements: Dean College of Pharmaceutical Sciences Ras Al Khaimah, Ras Al Khaimah Medical &Health Sciences University, Ras Al Khaimah, UAE. Microbiology department, Ras Al Khaimah Medical &Health Sciences University, Ras Al Khaimah, UAE. Professor Tim flower University Of Sussex School of Life Science Department Biology, John Maynard Smith Building, UK