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Tentative LC-MS-MS identification of flavonoids and phenolic acids from Scandiceae tribe (Apiaceae) Species
Tribe Scandiceae comprises 3 genera (Anthriscus, Chaerophyllum and Scandix) with over 60 species, the most of them considered weeds, although some are used as a food or in traditional medicine. The purpose of this work was to identify (by rapid resolution liquid chromatography coupled with electrospray-ionization triple-quad mass spectrometry) flavonoid and phenolic acids profile of 70% methanolic extracts of six species: A. sylvestris, A. cerefolium, Ch. bulbosum, Ch. hirsutum, Ch. temulentum and S. pecten-veneris. By means of targeted MS2 (Precursor Ion Scan) screening [1–3], it was possible to detect over 40 flavonoid O-glycosides, mostly hexosides, acetylhexosides and malonylhexosides of luteolin, apigenin, quercetin and methyl luteolin. While luteolin derivatives were present in all investigated species, the presence of other flavonoids was species-dependent and might serve as a chemotaxonomical marker. A number of chlorogenic acids (quinic acid esters with hydroxycinnamic acids) was also identified by MS2 and pseudo-MS3 experiments [4,5], including isomeric caffeoylquinic acids (CQA), dicaffeoylquinic acids (diCQA) and their acetyl-, malonyl-, and acetyl-malonyl-derivatives. The nature of chlorogenic acids was also species-dependent, with A. sylvestris being rich in unsubstituted CQA and diCQA, A. cerefolium and Ch. bulbosum having a high content of malonylated acids, and Ch. temulentum and S. pecten-veneris containing acetylated derivatives.
Acknowledgements: This work was supported by a research grant from the Ministry of Science and Technological Development, Republic of Serbia (Grant No. 142036).
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