Studies on the chemical constituents and their biological activities of the fruit of Garcinia multiflora

JJ Chen; CW Ting; MH Yen; TL Hwang; C Peng; IS Chen

doi:10.1055/s-0030-1264654

Planta Medica, Inhaltsverzeichnis

Studies on the chemical constituents and their biological activities of the fruit of Garcinia multiflora

JJ Chen ¹, CW Ting ², MH Yen ², TL Hwang ³, C Peng ⁴, IS Chen ²

¹Department of Pharmacy & Graduate Institute of Pharmaceutical Technology, Tajen University, No. 20, Weishin Rd., Yanpu Shiang, Pingtung 907, Taiwan
²Faculty of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan
³Graduate Institute of Natural Products, Chang Gung University, Taoyuan 333, Taiwan
⁴Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan

Abstract

Garcinia multiflora (Champ.) is a small evergreen tree of the Guttiferae family, distributed in South China, Hong Kong, and Taiwan [1]. Xanthones, flavonoids, benzophenones, and their derivatives are widely distributed in plants of the genus Garcinia. Many of these compounds exhibit cytotoxic, anti-inflammatory and antitubercular activities. In our studies on the anti-inflammatory constituents of Formosan plants, many species have been screened for in vitro anti-inflammatory activity, and G. multiflora has been found to be one of the active species. Six new benzophenone derivatives, 13,14-didehydoxyisogarcinol (1), garcimultiflorone A (2), garcimultiflorone B (3), 13-hydroxy-garcimultiflorone B (4), garcimultiflorone C (5), and garcimultiflorone D (6), together with eleven known compounds (7–17) have been isolated and identified from the fruit of G. multiflora. The structures of these new compounds were determined through spectral analyses including extensive 2D NMR. Among the isolates, garcimultiflorone B (3) exhibited potent inhibition with IC₅₀ values of 0.11±0.04 and 0.14±0.02µM, respectively, against fMLP/CB-induced superoxide anion generation and elastase release. In addition, δ-tocotrienol (10) showed the antitubercular activity with MIC value of 30.0µM against Mycobacterium tuberculosis H₃₇Rv in vitro.

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Acknowledgements: This work was supported by grant from the National Science Council of the Republic of China.

References: 1. Chang, C.E., Hartley, T.G. (1993) Guttiferae in Flora of Taiwan. 2nd edition. Editorial Committee of the Flora of Taiwan, Taipei, Taiwan 2: 694–714