The content of total phenolics and arbutin in some fruits from Bosnia
The aim of this study was to determine total phenolics with spectrophotometric method and arbutin using HPLC-ED method in the fruit extracts of blueberry, red currant, cowberry, sour cherry and wild cherry. The fruits were homogenized and extracted with water. Afterward of that extracts were centrifuged. Supernatants were used for futher analysis. The content of total phenols was estimated by a spectrophotometric method using gallic acid as a standard. HPLC-ED conditions: Mobile phase: EDTA, sodium acetate, acetic acid, methanol 50%, and water up to 1 L; ED detector with range 50 nA, potential +0.750 V, filter 0.02Hz; flow rate 0.9ml/min; temperature 25°C. Pure arbutin was dissolved in mobile phase and served as standard solution. It was estimated that total content of phenols in wild cherry was 7,5mg/g, in blueberry 5,5mg/g, in cowberry 5,1mg/g, red currant 2,7mg/g and sour cherry fruits had 2,2mg/g. The content of arbutin in blueberry was 21µg/g, red currant 26µg/g, cowberry 63µg/g and sour cherry 20µg/g. The content of arbutin in wild cherry was below limit of detection. All examined fruits contain uroantiseptic arbutin. The highest content of total phenolics was found in the fruits of wild cherry and blueberry.