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Quantitative HPLC analysis of rosmarinic acid and luteolin 5-O-β-glucoside in the aerial parts of Thymus praecox Opiz subs. großheimii (Ronniger) Jalas var. großheimii
There are 39 (64 taxa) Thymus species in Turkey, 27 taxa of which are endemic [1–3]. Thymus praecox subs. großheimii var. großheimii is used as a herbal tea for cold, stomachache, cough, infections in Erzurum Province (Turkey). In our previous study we have isolated two triterpenic acid compounds (ursolic acid and oleanolic acid), three phenolic compounds (rosmarinic acid, ethyl rosmarinate and methyl rosmarinate), one flavon glycoside (luteolin 5-O-β-glucoside), and one monoterpene diglycoside (thymoquinol 2,5-O-β-diglucopyranoside) from the aerial parts of the plant. In this study, the HPLC methods were applied for quantitative analysis of rosmarinic acid and luteolin 5-O-β-glucoside. Analysis were performed on an Agilent 1200 HPLC system, equipped with a DAD detector. For all analysis, RP-C18 column (250×4.6mm, 5µm particle size, ACE®) was used and maintained at 30°C. Solvents used for separation were 0.05% aqueous phosphoric acid (v/v) (A) and acetonitrile (B) for rosmarinic acid; 1% acetic acid (v/v) (A) and methanol (B) for luteolin 5-O-β-glucoside. Flow rate and sample volume were set to 1.0ml/min and 100µl, respectively. Detection wavelength was 330nm for rosmarinic acid, 350nm for luteolin 5-O-β-glucoside. The contents of the rosmarinic acid and luteolin 5-O-β-glucoside were 15.2mg/g and 57.8mg/g of dry weight, respectively.
Acknowledgements: The authors would like to thank Erzurum Regional Hygiene Institute for the technical support.
References: 1. Jalas J. (1982) Thymus L., in Flora of Turkey and the East Aegean Islands, Vol. 7, pp. 349–382, edited by P.H. Davis, University Press, Edinburgh, UK.
2. Davis P.H., Mill R.R., Kit T. (1988) Flora of Turkey and the East Aegean Islands, Vol. 10, pp. 209–504, University Press, Edinburgh, UK.
3. Güner A., Özhatay N., Ekim T., Baser K.H.C. (2000) Flora of Turkey and the East Aegean Islands, Vol. 11, p.209, University Press, Edinburgh, UK.