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Screening of sage and thyme plant extracts for some phenolic acids and rutin
Objectives: In this study, using HPLC-ED system, analysis of chlorogenic acid (CGA), gallic acid (GA), rosmarinic acid (RA) and caffeic acid (CA), and rutin was carried out in water extracts of sage and different thyme. Methods: Analyses of CGA, GA, RA, CA and rutin were performed of herbs sage and thyme. The drug (1g) was powdered and extracted with pure water (9ml). Afterward 1ml of that extract was decanted and centrifuged. Supernatant was used for analysis. The standard solutions were of CGA, GA, RA, and CA dissolved in mobile phase, and rutin was dissolved pure water. HPLC conditions were following: Mobile phase methanol-acetonitrile-water-acetic acid (20+10+70+1); ED detector with range 50nA, potential +0.840 V, filter 0.02Hz; flow rate 1ml/min; temperature 25°C, Column: ODS hypersil. Results: The content in (mg/g) of CGA was in the sage 0.36, GA 0.09, RA 5.45, CA 0.73, and rutin 11.8, thyme: (Thymus vulgaris L. CGA 1.21, GA 0.3, RA 4.27, CA 0.66, and rutin 24.9, Thymus serpyllum L. CGA 4.6, GA 0.95, RA 17.27, CA 1.36, and rutin 17.27, Thymus sibthorii Benth. CGA 1.44, GA 0.3, RA 5.5, CA 0.95, and rutin 22.64). Conclusion: The highest content of CGA, RA, CA and GA was determined in herbs of Thymus serpyllum L., and highest content of rutin was determined in Thymus vulgaris L.