Content of some phenolic acids and rutin in the leaves and roots of Symphytum officinale L.

Symphytum officinale L. is a perennial flowering plant of the genus Symphytum in the family Boraginaceae wich contains allantoin. Objectives: In this study, using HPLC-ED system, analysis of chlorogenic acid (CGA), gallic acid (GA), rosmarinic acid (RA) and caffeic acid (CA), and rutin was carried out in water extracts in the leaves and roots of S. officinale. Methods: Analyses of CGA, GA, RA, CA and rutin were performed in the leaves and root of S. officinale The drug (1g) was powdered and extracted with pure water (9ml). Afterward 1ml of that extract was decanted and centrifuged. Supernatant was used for analysis. The standard solutions were of CGA, GA, RA, and CA dissolved in mobile phase, and rutin was dissolved pure water. HPLC conditions were following: Mobile phase methanol-acetonitrile-water-acetic acid (20+10+70+1); ED detector with range 50nA, potential +0.840 V, filter 0.02Hz; flow rate 1ml/min; temperature 25°C, Column: ODS hypersil. Results: The content in (mg/g) of CGA was in the root of S. officinale 1.27, GA 0.05, RA 0.85, CA 0.15, and leaves GA 0.20, RA 1.15, CA 0.29. The content of CGA in leaves, and rutin in root was below limit of detection by this method. Conclusion: The highest content of RA, CA, GA and rutin was determined in leaves of S. officinale, while only was in roots determined CGA.