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Screening of 50 medicinal plant extracts for quercetin-3-rutinoside
The flavonoid rutin (quercetin-3-rutinoside), is a flavonol glycoside comprised of the flavonol quercetin and the disaccharide rutinose. Objectives: In this study, using HPLC-ED system, quantification of rutin was carried out in different extracts of medicinal plants. Methods: Analyses of rutin were performed on the leaves and flowers of 50 medicinal plants from Bosnia of rue, buckwheat, rose, sage, calendula mariogold, chamomile, elder, dandelion, feverfew, lemon balm, linden, thyme, valerian, stinging nettle, cloves, dog rose, pansy, parsley, cowslip, rose e.t.c. Rutin was extracted with hot water. Supernatant was used for analyses. The standard solution was rutin. HPLC conditions: Mobile phase methanol-acetonitrile-HPLC water-acetic acid (20+10+70+1); Potential: +0.840 V; Flow rate 0.8ml/min; Column: ODS hypersil. Results: Content of rutin (mg/g) was highest in the leaves of rue (86.0) and follow flowers of buckwheat (53.5), the leaves from buckwheat (20.0), flowers of pansy (33.5) and flowers of rose (10.0). In all other plants the content of rutin was lower than 0.5mg/g. The lowest content rutin found in the leaves lemon balm 0.25mg/g. Conclusion: The high concentration of rutin in flowers and leaves of rue, buckwheat, pansy and rose give more importance to rue, buckwheat, pansy and rose as medicinal and diet plants for theirs use to decreasing of capillary fragility.