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Quantification of rutin in some plants of family Lamiaceae using high performance liquid chromatography with electrochemical detection
Rutin is the glycoside of the flavonol quercetin and the disaccharide rutinose widely distributed in various medicinal plants. Objectives: In this study, using high performance liquid chromatography with electrochemical detection (HPLC-ED) system, quantitative analysis of rutin was carried out in water extracts of sage, mint, rosemary, thyme (Thymus vulgaris L., Thymus serpyllum L., and Thymus sibthorii Benth.), marjoram, oregano, and lemon balm. Method: The drug (1g) was powdered and extracted with pure water (9ml). Afterward 1ml of that extract was decanted and centrifuged. Supernatant was used for analysis. The standard solution was rutin dissolved in pure water. HPLC conditions were following: Mobile phase methanol-acetonitrile-water-acetic acid (20+10+70+1); ED detector with range 50nA, potential +0.840 V, filter 0.02Hz; flow rate 1ml/min; temperature 25°C. Results: The amounts of rutin in mg/g were: sage 11.8, mint 1.92, rosemary 1.61, thyme (Thymus vulgaris L. 24.9, Thymus serpyllum L. 9.0, Thymus sibthorii Benth. 22.64), marjoram 4.44, oregano 11.16, and lemon balm 0.25. Conclusion: The highest amount of rutin was in Thymus vulgaris L. following Thymus sibthorii Benth., and sage.