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Development and validation of an analytical method for the determination of derivatives of ortho-hydroxycinnamic acid of Echinodorus grandiflorus
Echinodorus grandiflorus (Cham. & Schltdl.) Micheli, a member of the family Alismataceae, is a native semi-aquatic plant widely distributed in Brazil, particularly in tropical regions of the country, and is popularly known as „chapéu-de-couro“ . This plant is used in folk medicine as an anti-inflammatory and diuretic . The chemical profile of E. grandiflorus consists basically of terpenoids, sesquiterpenes, and phenolic compounds [3–5]. A UV-Vis spectrophotometric method was developed and validated for the quantification of derivatives of ortho-hydroxycinnamic acid in leaves of E. grandiflorus. The method was validated by regulation RE 899/2003 of the National Health Surveillance Agency, Brazil, and the ICH guidelines. The response of this method was linear in concentrations ranging from 0.25–0.90µg mL–1 with a linear-regression correlation coefficient of 0.9972. The content of derivatives of ortho-hydroxycinnamic acid was successfully determined, with satisfactory reproducibility and recovery. The recovery was 107.56%. This method fulfilled all the validation parameters and was rapid, feasible, and low-cost. Thus, it can be applied by dispensing pharmacies and small laboratories for routine quality control analysis of E. grandiflorus.
Acknowledgements: ANVISA (Agência Nacional de Vigilância Sanitária, Brazil) and Brazilian Pharmacopoeia.
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