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Antioxidant homoisoflavonoids from Chionodoxa forbesii Baker
Chionodoxa is a genus within the Hyacinthaceae, which species are high in homoisoflavonoids (HFs) . Antibacterial, anti-mutagenic and anti-inflammatory effects have been described for some HFs, especially for those from Eucomis and Scilla . A detailed examination of HFs from Chionodoxa forbesii Baker (syn. Ch. gigantea Whittall) is missing in literature, thus the aim of this work was to determine its compounds. A mixture of substances yielded by extraction with ethanol and ether was separated by column chromatography on silica gel and polyamide. The compounds were identified using FT-IR, ESI-MS and 1H- NMR and 13C-NMR. Thereby, HFs of the 3-benzyl-4-chromone-, 3-benzyl-3-hydroxy-4-chromanone and the scillascillin-type were determined. The pattern of substances was found to be in strong relation to this of Ch. luciliae Boiss. . For measuring the antioxidant potentials the ABTS radical decolourization assay following the method described by Re et al.  and the DPPH free radical scavenging method were used. Ascorbic acid and trolox served as references. Scillascillin-type HFs showed low activity, whereas 3-benzyl-4-chromone-type HF had a high potential. Cytotoxic effects of HFs on human cells were tested using trypan blue vital stain on HEK-293 cells. It was found, that the tested HFs do not show any adverse cytotoxic effects.
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