Planta Med 2010; 76 - P243
DOI: 10.1055/s-0030-1264541

Antioxidant homoisoflavonoids from Chionodoxa forbesii Baker

F Klar 1
  • 1Flurepha, Division of Natural Product Research, Buer-Gladbecker Str. 78, 45894 Gelsenkirchen, Germany

Chionodoxa is a genus within the Hyacinthaceae, which species are high in homoisoflavonoids (HFs) [1]. Antibacterial, anti-mutagenic and anti-inflammatory effects have been described for some HFs, especially for those from Eucomis and Scilla [2]. A detailed examination of HFs from Chionodoxa forbesii Baker (syn. Ch. gigantea Whittall) is missing in literature, thus the aim of this work was to determine its compounds. A mixture of substances yielded by extraction with ethanol and ether was separated by column chromatography on silica gel and polyamide. The compounds were identified using FT-IR, ESI-MS and 1H- NMR and 13C-NMR. Thereby, HFs of the 3-benzyl-4-chromone-, 3-benzyl-3-hydroxy-4-chromanone and the scillascillin-type were determined. The pattern of substances was found to be in strong relation to this of Ch. luciliae Boiss. [3]. For measuring the antioxidant potentials the ABTS radical decolourization assay following the method described by Re et al. [4] and the DPPH free radical scavenging method were used. Ascorbic acid and trolox served as references. Scillascillin-type HFs showed low activity, whereas 3-benzyl-4-chromone-type HF had a high potential. Cytotoxic effects of HFs on human cells were tested using trypan blue vital stain on HEK-293 cells. It was found, that the tested HFs do not show any adverse cytotoxic effects.

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