Planta Med 2010; 76 - P232
DOI: 10.1055/s-0030-1264530

Cynoglossum columnae Ten. – chemical profiling through biotechnological and phytochemical approach

M Jeziorek 1, H Damianakos 2, A Pietrosiuk 1, G Sotiroudis 3, W Buchwald 4, K Syklowska-Baranek 1, I Chinou 2
  • 1Medical University of Warsaw, Department of Biology and Pharmaceutical Botany, Banacha 1, 02097 Warsaw, Poland
  • 2University of Athens, School of Pharmacy, Department of Pharmacognosy, University Campus of Zografou, 157 71 Zografou Athens, Greece
  • 3National Research Institute, Institute of Biological Research and Biotechnology, Vas. Konstantinou 48, 11635 Athens, Greece
  • 4Institute of Natural Fibres and Medicinal Plants, The Branch of Medicinal Plants, Libelta 27, 61–707 Poznan, Poland

Cynoglossum columnae Ten. (Boraginaceae) is an annual species of Mediterranean region. Plants of this family are investigated for naphthoquinone red pigments, found in the underground parts, which are bioactive constituents known as: wound healing, anti-inflammatory, antimicrobial, antitumor agents [1]. Boraginaceae family is also known for its content of hepatotoxic pyrrolizidine alkaloids (PAs) [2]. Three groups of in vitro root cultures were obtained and investigated: the natural roots cut off from seedlings, natural roots regenerated from shoots in the following passages and hairy roots obtained as a result of transformation with Agrobacterium rhizogenes– ATCC 15834 strain. The roots were cultured in various liquid media (ex.: MS, B5, LS, DCR) and tested for their growth and production of naphthoquinones. The best results gave DCR medium [3] with twice reduced amount of all components and full sugar value. Preliminary phytochemical analysis was performed using RP-HPLC DAD method and showed the presence of six naphtoquinone derivatives in natural roots and their post culture media (DCR/2); none in the transformed root cultures was observed. Phytochemical investigation in plants cultivated in nature was made and six PAs have been isolated and determined by modern spectroscopic methods as rinderine, 3′-acetylrinderine and echinatine in the form of both their N-oxides as well as their bases after reduction. Sixteen more, known PAs, were also identified after reduction as echimumiline, heliosupine, heliotridine, echinatine, rinderine, retronecine, integerrimine and triangularicine type derivatives.

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2. Chojkier M. (2003)J Hepatol 39: 437–446.

3. Gupta P. K., Durzan D. J. (1985) Plant Cell Rep. 4:177–179.