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DOI: 10.1055/s-0030-1264529
Pro-apoptotic effects of lowbush blueberry proanthocyanidins in human colon carcinoma SW480 and SW620 cells
Proanthocyanidins (Pcy) are oligomeric flavan-3-ols with strong anti-cancer properties described in different models [1]. We have previously demonstrated in vitro as well as in a rat model of colon carcinogenesis, the potential of apple Pcy in colorectal cancer chemoprevention [2–4].
We have performed by microcapillary cytometry a high content screening assay on the ability of Pcy-rich extracts to induce apoptosis in metastatic human colon cancer cell line (SW620). Here, we focused our interest on lowbush blueberry (LB) (Vaccinium angustifolium Aiton, Ericaceae) and demonstrated that LB Pcy were more potent to induce apoptosis than apple Pcy, our internal standard, both on SW620 and SW480 cell lines (see table).
% apoptosis |
|||
Sample (75µg/ml) |
SW480 cells |
SW620 cells |
% flavan-3-ols |
Apple Pcy-rich extract |
55.3±9.4 |
64±8.9 |
150.6±5.7 |
LB Pcy-rich extract |
92.3±6.1 |
94.9±1.3 |
101.7±1.2 |
No synergy was observed between LB Pcy and TNF-α, and no effect of LB Pcy on surface death receptors expression in both cell lines was found. So, the hypothesis of a death receptor clustering was verified by immunocytochemistry.
In order to highlight the chemopreventive potential of LB Pcy in CCR, the intracellular mechanisms of action, as well as the in vivo efficacy of an LB standardized Pcy-extract, are being currently studied.
References: 1. Nandakumar V et al., Cancer Letters (2008); 269: 378–387.
2. Gossé F et al., Carcinogenesis (2005); 26(7): 1291–1295.
3. Seiler N et al., Anticancer Res. (2006); 26: 3381–3386.
4. Maldonado-Celis ME et al., Cell. Mol. Life Sci. (2008); 65(9): 1425–1434.