Phytoecdysteroids from arial parts of Ajuga chamaecistus subsp. tomentella
The genus Ajuga (Lamiaceae) is represented by five species in the flora of Iran, of which Ajuga chamaecistus has several exclusive subspecies include subsp. tomentella . The plants of this genus are used traditionally for treatment of joints pain, gout and jaundice . In this study diethylether fraction of defatted methanolic extract (80%) from arial parts of A. chamaecistus subsp. Tomentella was chromatographed on silicagel using a CHCL3-EtoAc-MeOH gradient system to give compound 1 and 2. The structure of compound 1 and 2 were determined to be 20-Hydroxyecdysone and Cyasterone, respectively, by means of spectroscopic analysis (13C, HNMR, IR, Mass Spectroscopy). Ecdysteriods are a large group of natural polyhydroxysteroids with a wide spectrum of biological activities, which are isolated from the animal and plant kingdoms . In plants of genus Ajuga, a variety of phytoecdysteroids has been isolated. Among them, 20-Hydroxyecdysone or Cyasterone are usually the most abundant 
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