Planta Med 2010; 76 - P062
DOI: 10.1055/s-0030-1264360

Vanda coerulea stem's bio-markers inhibit COX-2 and prevent UV-induced MMP-9 and pro MMP-1 production in skin cells

C Simmler 1, V Leplanquais 2, P André 2, F Bonté 3, A Lobstein 1
  • 1Faculty of Pharmacy University of Strasbourg, UMR 7200, laboratory of pharmacognosy, 74 route du rhin, 67400 Illkirch, France
  • 2LVMH recherche, Departement Innovation Actifs, 185 avenue de verdun, 45800 Saint Jean de Braye, France
  • 3Guerlain, 125 rue du Président Wilson, 92290 Levallois Perret, France

Background: According to our previous results [1], imbricatin (1) and methoxycoleonin (2), the two major dihydrophenanthrenes isolated from V. coerulea Griff ex. Lindl (Orchidaceae) stems, displayed radical scavenging activities and inhibited PGE-2 release from UVB (60mJ/cm2) irradiated human normal epidermal keratinocytes (NHEK). It has been reported that COX-2, which synthetised PGE-2, could be an effective target for the regulation of UV-induced skin disorders. Besides, UVB radiation promotes the degradation of dermal extracellular matrix (ECM) by matrix metalloproteinases (MMPs) [2]. Principal findings: To get further insights in the anti-inflammatory and skin protection effects of these bio-markers, we have measured their activities on COX-2 and on UV-induced MMP-9/pro-MMP-1 production on NHEK and Human Normal Dermal Fibroblast (HNDF), respectively. Quantification of PGE-2 produced was considered as an indicator of human recombinant COX-2 enzyme activity. UV induced MMP-9 and pro-MMP-1 released by irradiated NHEK and HNDF were quantified by Elisa. Compounds (1) and (2) were able to inhibit significantly COX-2 (IC50 12.0 and 5.8µM, respectively) when compared with positive reference indomethacin (1.6µM). Besides, they were able to prevent cellular release of UV-induced pro MMP-1(IC50 6.7 and 9.4µM, respectively) and MMP-9 (IC50 2.8 and 2.6µM, respectively) in a dose dependant manner. Conclusions: PGE-2, implied in skin inflammation, is also known to trigger the release of MMPs, promoting dermal matrix damages but also cellular proliferation [3]. So, the antioxidant dihydro-phenanthrenes isolated from V. coerulea stems displayed complementary skin care and anti ageing effects by inhibiting PGE-2 and major MMPs production from skin cells.

References: 1. Simmler C et al (2009) Planta Med.

2. Rittié L et al. (2002) Aging Res Rev 3. Tripp CS et al (2003)J Invest Dermatol.