Planta Med 2010; 76 - P050
DOI: 10.1055/s-0030-1264348

Triterpenoidal saponins from Hydrocotyle bonariensis

T Tabopda Kuiate 1, A Mitaine-Offer 1, T Miyamoto 2, B Ngadjui Tchaleu 3, M Lacaille-Dubois 4
  • 1University of Burgundy, Faculty of Pharmacy, Laboratory of Pharmacognosy, EA 3660, 7, Bd Jeanne D'Arc, 21079 Dijon, France
  • 2Kyushu University, Graduate School of Pharmaceutical Sciences, Maidashi Higashi Ku 3–1-1, 812–8582 Fukuoka, Japan
  • 3Université de Yaoundé 1, Département de chimie organique, BP 812, 30000 Yaoundé, Cameroon
  • 4University of Burgundy, Faculty of Pharmacy, Laboratory of Pharmacognosy, EA 3660, Pharmacognosy, UMIB, EA 3660, 7, Bd Jeanne d'Arc, 21079 Dijon, France

The genus Hydrocotyle is an aquatic or semi-aquatic plant having antioxidant and antitumoral activity [1, 2]. Earlier investigations have reported on a therapeutic action on ulcer, wounds, eczemas, and antitumour activity of compounds from Hydrocotyle species [3, 4]. This genus is particularly well represented in temperate areas and higher tropical region [5]. There are very rich in saponins, which have many interesting biological activities such as anti-inflamatory, antimicrobial, molluscicidal, cytotoxic, and antitumor properties [6]. In view of the biological and pharmacological importance of this type of secondary metabolites, we have focused our intention in the study of H. bonariensis. It is a hairless and creepy perennial plant. In the present research, we describe the isolation and structure elucidation of six new oleanane-type triterpenoidal saponins, Bonarienosides A-F (1 - 6) from the 70% aqueous MeOH extract of Hydrocotyle bonariensis roots. Their structures were established on the basis of NMR spectroscopic data (13C, 1H, COSY, HMBC, TOCSY, and NOESY) and mass spectrometry.

Acknowledgements: The authors are grateful to the Conseil Régional de Bourgogne, France for financial support.

References: 1. Yu, F. et al. (2007) Phytomed. 14: 166–171.

2. Jayashree, G. et al. (2003) Fitoterapia 74: 431–434.

3. Malhotra, C.L. et al. (1961) Ind. J. Pharm. 23: 106.

4. Miyata, S. et al. (1981) Anticancer Crude Drugs and their Prescription 185.

5. Stevens, C. et al. (2000) Missouri Botanical Garden Press. 110–115.

6. Lacaille-Dubois, M.A., Mitaine-Offer A.C. (2005) Phytochem. Rev. 4: 139–149.