The first report of microsatellite primer pairs for genetic studies in jojoba [Simmondsia chinensis (Link) Schneider

Jojoba [Simmondsia chinensis (Link) Schneider] is an important industrial plant, native to Arizona, southern California and northern Mexico. The product of primary interest in jojoba is the seed oil, which consists of esters formed from acids and alcohols with chain lengths of 20 or 22 carbon atoms. As many as 300 products containing jojoba have appeared in markets in recent years and the use of jojoba products is expected to increase in future [1]. The use of DNA markers in jojoba breeding is limited and the best method for jojoba improvement has been the selection of plants with desirable characteristics. DNA markers have been extensively used in plant improvement studies [2,3,4]. In the present study a total of 10 microsatellite markers were identified using a strategy described in [5] and this is the first report on jojoba microsatellite markers. In order to evaluate microsatellite primer pairs (Table 1), genomic DNAs of several jojoba samples were extracted using a DNA extraction protocol described in [6]. Results indicated that these primer pairs are very useful in genetic studies of jojoba.

Table 1: List of microsatellite primer pairs and related information

Locus	Forward Primer (5→ 3′)	Reverse Primer (5′→ 3′)	Motif	Size (bp)
JMA01	ACACCAGATTCCAGAGGCATA	ATTCGTCAAAGGGGATGATG	[CT]8	198
JMA02	AGAGTACGCGGGAAGCAGT	TGCTGGCAAGGGAGGTAATA	[AG]8	600
JMA03	AGTCGTTTCCCCTGCTTTTC	CTTCTGCTTATCCCCCTCATC	[CT]7	320
JMA04	GGACCTCTGCCCTTCTTCTT	TGGCGTCTTCACTGCTACTC	[GT]11	500
JMA05	CGGGGATTTATAGTCTTCACTCTC	GTCCAGGCTTCAGACCAGAG	[TC]13	214
JMA06	GCATCTGCCATTTTATGTTCAG	AACCCAGTTCCAGCTTCATC	[AAT]5	180
JMA07	GCCAAGTGGGGATGTAGAGA	GGGGACTGAACTCCACCAA	[GA]8	165
JMA08	GGAACCACAATGGCAACG	CGCAGGAAGGTCGTAAACTG	[TCT]9	185
JMA09	GCGGGGAAAGTGTTACGC	GATTAGCAGAGAAACCAAGGGACT	[AG]15	190
JMA10	AGTCAGAGTCACAGAGCAATGAA	AAGAGATTAGCAGAGAAACCAAGG	[TCT]5	700

References: 1. Ince, A.G. et al. (2009) Genet. Resour. Crop. Ev. doi: 10.1007/s10722–009–9516–1.

2. Karaca, M., Ince, A.G. (2008)J. Genet. 87:83–86.

3. Ince, A.G. et al. (2009) Genet. Resour. Crop. Ev. 56:211–221.

4. Ince, A.G., Karaca, M. (2009)J. Sci. Food Agric. 89:168–176.

5. Ince, A.G. et al. (2008) Plant Cell Tissue Organ Cult. 94:281–290.

6. Karaca, M. et al. (2005) Anal. Biochem. 343:353–355.