Planta Med 2010; 76 - SL_43
DOI: 10.1055/s-0030-1264281

HPTLC method development and validation for estimation of isovitexin from methanolic extract of Enicostemma littorale Blume

L Sawant 1, B Prabhakar 1, N Pandita 1
  • 1School of Pharmacy & Technology Management, SVKMS NMIMS University, Pharmaceutical chemistry, Senior Research fellow, School of Pharmacy & Technology Management, SVKMS NMIMS University, V. L. Mehta road, Vile parle (West), Mumbai 400056, India, 400056 Mumbai, India

Enicostemma littorale Blume (Chota-Kirayat or Chota-Chirayata) is a glabrous perennial herb belongs to family Gentianaceae [1]. It is used as anti-diabetic, anti-inflammatory, anticancer and antioxidant [2, 3]. In the present study, a new, simple, sensitive, precise and robust high-performance thin layer chromatographic (HPTLC) method was developed and validated for the estimation of isovitexin from methanolic extract of E. littorale. Analysis of isovitexin was performed on TLC aluminium plates pre-coated with silica gel 60 RP-18 F-254 as stationary phase. Linear ascending development was carried out in twin trough glass chamber saturated with mobile phase consisting of acetonitrile-water (3:7, v/v). Camag TLC scanner III was used for spectrodensitometric scanning and analysis of the plate in absorbance mode at 235nm. The system was found to give compact spots for isovitexin (Rf value of 0.40). The data for calibration plots showed good linear relationship with r2=0.99853 in the concentration range of 50–400 spot-1 with respect to peak area. The present method was validated for precision and recovery. The total isovitexin content was found to be 0.4% (w/w) in methanolic extract of E. littorale. Statistical analysis proves that the method is reproducible and selective for the analysis of isovitexin.

References: 1. Murali, B. et al. (2002)J. Ethnopharmacol. 81:199–204.

2. Maroo, J. et al. (2003) Phytomed. 10:196–199.

3. Sadique, J. et al. (1987) Biochem Med Metabol Biol. 37:167–176.