Isolation of differencially expressed genes from Psoralea corylifolia by DD-PCR

According to the world health organization (WHO) 80% of the world's population uses medicinal plants for the treatment of diseases. In recent years medicinal plants are primary health source for pharmaceutical industry. Psoralea corylifolia (Psoralea seed) is used in treatment of many skin diseases in Pakistan and India. Development of resistance in fungi to commonly used antifungal drugs diverted the attention of researches towards medicinal plants. In the present study we focused our research towards isolation of differenzially expressed genes from seedlings of Psoralea corylifolia after induction with a fungus, Fusarium solani. RNA was isolated from control (non-induced) and fungal induced seedlings. Quantity and integrity of RNA was checked by agarose gel electrophoresis and spectroscopy. cDNA was formed from RNA by reverse transcription using oligo-dT primers. All PCR reactions contained the same T₁₁MN primer, and an arbitrary primer. Different arbitrary primers (HAP 25–32) were tried for each cDNA. The amplified products were resolved on 6% denaturing polyacrylamide gel electrophoresis and detection was carried out with silver staining. Differenzially expressed genes were isolated from the induced samples after comparing with the controls after sequencing.