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Synfacts 2011(7): 0781-0781
DOI: 10.1055/s-0030-1260636
DOI: 10.1055/s-0030-1260636
Organo- and Biocatalysis
© Georg Thieme Verlag
Stuttgart ˙ New York
Biosynthesis of Spinosyn A
H. J. Kim, M. W. Ruszczycky, S.-H Choi, Y.-N. Liu, H.-W. Liu*
University of Texas at Austin, USA
Further Information
Publication History
Publication Date:
17 June 2011 (online)
Significance
The authors report a detailed study on the biosynthesis of spinosyn A, an important insecticide from the bacterium Saccharopolyspora spinosa. By systematically investigating the enzymes from the spinosyn A biosynthetic gene cluster overexpressed from Escherichia coli, two important cyclases were identified. The roles of these two enzymes, SpnF and SpnL, are the catalysis of a Diels-Alder reaction and a Rauhut-Currier cyclization, respectively. The Diels-Alder reaction was shown to undergo a 500-fold rate enhancement compared to the non-catalyzed reaction.