Probiotic treatment of intestinal flora lesion caused by antibiotic therapy

Introduction: Diarrhoea develops in about 5–40% of in-patients treated with antibiotics. It is possible to apply probiotics instead of antibiotics to treat and prevent this type of infections. Aim: Our aim was to examine what kind of probiotic micro-organism is beneficial in the prevention of antibiotic associated diarrhoea and find out whether antibiotics destroy the probiotics. Does the probiotics affect proliferation of bacteria causing gastrointestinal infections and development of infections in vitro? Materials and Methods: We analyzed the effect of B. clausii, L. acidophilus LA-5, L. bulgaricus, B. Bifidum BB-12 probiotical bacteria on proliferation of pathogen bacteria and pathogen proliferous fungi in vitro. Determination of antibiotic sensitivity was carried out with Kirby bauer agar diffusion method. Results: B. clausii blocked Shigella growth of and had bacteriostatic effect on S. aureus and E. faecalis. L. delbruecki isolated from kefir blocked the proliferation of shigella and salmonella and was bacteriostatic to S. aureus. Neither probiotics obstructed the proliferation of P. mirabilis, ETEC, EHEC, E. sp. and MRSA. B. BB-12 blocked the growth of C. glabrata and destroyed it partially in vitro; it was fungistatic to C. albicans, and had no effect on growth of C. parapsilosis and C. kruzei. Conclusions: On the basis of our results B. clausii is well applicable in macrolide antibiotic treatments, and penicillin and tobramycin therapies so that the antibiotic does not destroy it with the pathogen causative agents. In case of use sumetrolim, kefir and joghurt with lactobacilluses are suggested. Lactobacilli were moderately sensitive to cefeprim, ceftazidin, but we can advise products which contain a high number of these germs. B. BB-12 was totaly resistant to methronidazol, erythromycin, it was reactive to the other antibiotics. It can be applied only in case of metronidazol or vancomycin therapy of diarrhoea caused by C. difficile. Products containing L. acidophilus LA-5 and B. BB-12 can also be used with every antibiotic to witch one or other probiotics are resistant.