Marker Compounds from the Herb Lancea tibetica and Identification by HPTLC

Lancea tibetica Hook. F. et Thoms, a Tibetan medicinal plant, is traditionally used for the prevention and treatment of leukemia, intestinal angina, heart disease and cough by the Zang people [1]. Sylvatesmin has been reported to have anti-tumor and anti-oxidant activity in vitro [2,3]. A phytochemical investigation led us to isolate and identify 15 compounds from this plant, including furofuranoliganans, phenylpropanoid glycosides, triterpenes and iridoid glycosides. Eleven compounds, including 7 furofuranoliganans, verbascoside, isoverbascoside, β-sitosterol and oleanolic acid were used as the marker compounds to identify this plant by HPTLC method. Three of these furofuranoliganans (shown in Fig.1) (tibeticoside, lantibeside, and lantibeside C) have only been reported from this plant. A fast and reliable HPTLC method was developed for the quality analysis of this widely used Tibetan medicine. The HPTLC profiles are shown in Fig.2 and Fig.3. The developed method was successfully applied for the identification of 5 Lancea tibetica samples collected from different regions in China. The developed method was validated for specificity, stability, repeatability, and robustness.

Acknowledgments: We thank Dr. Yue-Guo Zhong from Chongqing Research Institute of TCMs for the assistance in collecting and identifying Lancea tibetica samples. This research is supported in part by „Science Based Authentication of Dietary Supplements“ and „Botanical Dietary Supplement Research“ funded by the Food and Drug Administration grant numbers 5U01FD002071–09 and 1U01FD003871–01, and the United States Department of Agriculture, Agricultural Research Service, Specific Cooperative Agreement No. 58–6408–2-0009. References: [1] Chinese Herbs Editor. Zhonghua Bencao (Volume of Tibetan Medicine)(in Chinese). Shanghai: Shanghai Science and Technology Press (2002): 192. [2] Li T, Hao XJ, et al. (2008) Planta Medica, 74: 1391–1394. [3] Miao JL, Wang WF, et al. (2004) Radiation Physics and Chemistry 69: 25–29.

P58, Fig.1: Marker compounds isolated from the herbs of Lancea Tibetica

P58, Fig.2: HPTLC Profile of the herbs of Lancea Tibetica

P58, Fig.3: HPTLC Profile of the herbs of Lancea Tibetica