© Georg Thieme Verlag KG Stuttgart · New York
Anti-Inflammatory and Anti-Melanogenic Steroidal Saponin Glycosides from Fenugreek (Trigonella foenum-graecum L.) Seeds
received April 6, 2010
revised October 1, 2010
accepted October 4, 2010
26 October 2010 (eFirst)
Fenugreek seed (Trigonella foenum-graecum L.) is used as an herbal medicine for treating metabolic and nutritive dysfunctions. To determine if this plant has other beneficial effects, we tested the inhibitory activities of a methanol (MeOH) extract of fenugreek seed on the production of inflammatory cytokines and melanin synthesis in cultured cell lines in vitro. The MeOH extract inhibited the production of phorbol-12-myristate-13-acetate-induced inflammatory cytokines such as tumor necrosis factor (TNF)-α in cultured THP-1 cells, and also restrained the intracellular synthesis of melanin in murine melanoma B16F1 cells. We isolated three active constituents from fenugreek seed extracts. These were identified as the steroidal saponins 26-O-β-D-glucopyranosyl-(25R)-furost-5(6)-en-3β,22β,26-triol-3-O-α-L-rhamno-pyranosyl-(1′′→ 2′)-O-[β-D-glucopyranosyl-(1′′′ → 6′)-O]-β-D-glucopyranoside 1, minutoside B 2, and pseudoprotodioscin 3. Compounds 1 and 2 strongly suppressed the production of inflammatory cytokines, whereas 3 showed a weaker suppressing effect. Melanogenesis in B16F1 cells was significantly suppressed by 1 and 3, and weakly suppressed by 2. All three compounds showed moderate cytotoxicities. These results indicate that fenugreek extract and its active constituents could protect against skin damage.
Trigonella foenum‐graecum - Fabaceae - anti‐inflammatory - melanogenesis - steroidal saponin