Planta Med 2011; 77(3): 256-258
DOI: 10.1055/s-0030-1250383
Biological and Pharmacological Activity
© Georg Thieme Verlag KG Stuttgart · New York

Inhibitors of Nitric Oxide Production from Stemona javanica

Takahiro Hosoya1 , Fumie Yamasaki1 , Asami Nakata1 , Abdul Rahman2 , Idha Kusumawati2 , Noor Cholies Zaini2 , Hiroshi Morita1
  • 1Faculty of Pharmaceutical Sciences, Hoshi University, Shinagawa, Tokyo, Japan
  • 2Faculty of Pharmacy, Airlangga University, Jalan Dharmawangsa Dalam, Surabaya, Indonesia
Further Information

Publication History

received May 1, 2010 revised July 22, 2010

accepted September 1, 2010

Publication Date:
01 October 2010 (online)


In our screening program for bioactive natural products from our library of tropical plants, the extract prepared from the roots of Stemona javanica inhibited NO production in mouse macrophage-like cell line J774.1 stimulated by lipopolysaccharide (LPS). Bioassay-guided fractionation of the extract from S. javanica led to the isolation of two active compounds, stemofoline (1) and stemanthrene C (2). The inhibition mechanism of 1 was proposed to suppress iNOS expression in J774.1 cells stimulated by LPS, whereas that of 2 was due to potent radical scavenging activity resulting in NO inhibitory activity.


Prof. Dr. Hiroshi Morita

Department of Pharmacognosy
Faculty of Pharmaceutical Sciences
Hoshi University


Tokyo 142-8501


Phone: +81 3 54 98 57 78

Fax: +81 3 54 98 57 78