Planta Med 2011; 77(2): 117-121
DOI: 10.1055/s-0030-1250235
Biological and Pharmacological Activity
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Inhibitory Effects of Dendrobium Alkaloids on Memory Impairment Induced by Lipopolysaccharide in Rats

Yanfei Li1 , Fei Li1 , Qihai Gong1 , Qin Wu2 , Jingshan Shi1 , 2
  • 1Department of Pharmacology, Zunyi Medical College, Zunyi, P. R. China
  • 2The Key Laboratory of Basic Pharmacology of Guizhou, Zunyi, P. R. China
Further Information

Publication History

received April 1, 2010 revised June 8, 2010

accepted July 9, 2010

Publication Date:
17 August 2010 (online)

Abstract

Dendrobium alkaloids (DNLA), extracted from Dendrobium nobile Lindl. whose botanical name is Dendrobium moniliforme, Orchidaceae family, were studied for their effect on lipopolysaccharide (LPS)-induced memory impairment in rats. SD rats were pretreated with DNLA (40, 80, 160 mg/kg/d for 7 d), followed by LPS (50 µg) injection into the right lateral ventricle to produce memory impairment. DNLA treatment continued for another 13 days. The spatial behavior was tested by the Morris water maze; the level of tumor necrosis factor receptor 1 (TNFR1) mRNA was detected by real time RT‐PCR, and the protein level of TNFR1, nuclear factor kappa-B (NF-κB) and phosphorylated p38 mitogen-activated protein kinases (p-p38 MAPK) by Western blotting. The results showed that DNLA significantly improved the neurobehavioral performance and prevented LPS-induced elevation in TNFR1 mRNA and protein levels. LPS-induced activation of p38 MAPK and NF-κB pathway was also suppressed. In conclusion, DNLA is effective in protecting against LPS-induced brain impairment, and this effect is due, at least in part, to prevent overexpression of TNFR1 via inhibition of p-p38 MAPK and the downstream NF-κB signal pathway.

References

Dr. Jingshan Shi

Department of Pharmacology
Zunyi Medical College

120 Dalian Road

Zunyi, Guizhou 563003

P. R. China

Phone: + 86 85 28 60 97 88

Fax: + 86 85 28 60 95 75

Email: [email protected]