Abstract
In this study, we tested the applicability of four DNA regions (rbcL, matK, ITS, and ITS2) as barcodes for identifying species within Euphorbiaceae. Based on
assessments of the specific genetic divergence, the DNA barcoding gap, and the ability
for species discrimination, the present results affirmed that ITS/ITS2 is a potential
barcode for the Euphorbiaceae species. This study also provided a large-scale test
to evaluate the effectiveness of ITS/ITS2 for differentiating species within Euphorbiaceae.
Of the 1183 plant samples collected from 871 species in 66 diverse genera, ITS/ITS2
successfully identified > 90 % and 100 % of them at the species and genus levels,
respectively. Therefore, our research indicates that use of the ITS/ITS2 region is
a powerful technique for Euphorbiaceae identification.
Key words
DNA barcoding - species identification - Euphorbiaceae - intra‐/interspecific genetic
divergence
References
- 1
Heinrich M.
The identification of medicinal plants.
J Ethnopharmacol.
2007;
111
440
- 2
Hebert P D N, Cywinska A, Ball S L, de Waard J R.
Biological identifications through DNA barcodes.
Proc Biol Sci.
2003;
270
313-321
- 3
Moritz C, Cicero C.
DNA barcoding: promises and pitfalls.
PLoS Biol.
2004;
2
1529-1534
- 4
Hebert P D N, Gregory T R.
The promise barcoding for taxonomy.
Syst Biol.
2005;
54
852-859
- 5
Evans K M, Wortley A H, Mann D G.
An assessment of potential diatom “barcode” genes (cox1, rbcL, 18S and ITS rDNA) and their effectiveness in determining relationships in Sellaphora (Bacillariophyta).
Protist.
2007;
158
349-364
- 6
Lahaye R, van der Bank M, Bogarin D, Warner J, Pupulin F, Gigot G.
DNA barcoding the floras of biodiversity hotspots.
Proc Natl Acad Sci USA.
2008;
105
2923-2928
- 7
CBOL Plant Working Group .
A DNA barcode for land plants.
Proc Natl Acad Sci USA.
2009;
106
12794-12797
- 8
Chen S L, Yao H, Han J P, Liu C, Song J Y, Shi L C, Zhu Y J, Ma X Y, Gao T, Pang X H,
Luo K, Li Y, Li X W, Jia X C, Lin Y L, Leon C.
Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant
species.
PLoS ONE.
DOI: 10.1371/journal.pone.0008613
, advance online publication 7 January 2010
- 9
Wilson S R, Neubert L A, Huffman J C.
The chemistry of the Euphorbiaceae. A new diterpene from Croton californicus.
J Am Chem Soc.
1979;
98
3669-3674
- 10
Agra M F, França P F, Barbosa-Filho J M.
Synopsis of the plants known as medicinal and poisonous in Northeast of Brazil.
Rev Bras Farmacogn.
2007;
17
114-140
- 11
Yadav R P, Singh D, Singh S K, Singh A.
Metabolic changes in freshwater fish Channa punctatus due to stem-bark extract of Croton tiglium.
Pakistan J Biol Sci.
2003;
6
1223-1228
- 12
Rodriguez J A, Hiruma-Lima C A.
Antiulcer activity and subacute toxicity of trans-dehydrocrotonin from Croton cajucara.
Hum Exp Toxicol.
2004;
23
455-461
- 13
Antonio S, Maria L, Faria S, Giuseppina N.
Traditional uses, chemistry and pharmacology of Croton species (Euphorbiaceae).
J Braz Chem Soc.
2007;
18
11-33
- 14
Tagwireyi D, Ball D E, Loga P J, Moyo S.
Cantharidin poisoning due to “blister beetle” ingestion.
Toxicon.
2000;
12
1865-1869
- 15
Meyer C P, Paulay G.
DNA barcoding: error rates based on comprehensive sampling.
PLoS Biol.
2005;
3
2229-2238
- 16
Alvarez I, Wendel J F.
Ribosomal ITS sequences and plant phylogenetic inference.
Mol Phylogenet Evol.
2003;
29
417-434
- 17
Feliner G N, Rosselló J A.
Better the devil you know? Guidelines for insightful utilization of nrDNA ITS in species-level
evolutionary studies in plants.
Mol Phylogenet Evol.
2007;
44
911-919
- 18
Chase M W, Cowan R S, Hollingsworth P M, van den Berg C, Madriñán S, Petersen G, Seberg O,
Jørgsensen T, Cameron K M, Carine M.
A proposal for a standardised protocol to barcode all land plants.
Taxon.
2007;
56
295-299
- 19
Kress W J, Erickson D L.
A two-locus global DNA barcode for land plants: the coding rbcL gene complements the non-coding trnH-psbA spacer region.
PLoS ONE.
2007;
2
e508
- 20
Coleman A W.
Pan-eukaryote ITS2 homologies revealed by RNA secondary structure.
Nucleic Acids Res.
2007;
35
3322-3329
- 21
Miao M, Warren A, Song W, Wang S, Shang H, Chen Z.
Analysis of the internal transcribed spacer 2 (ITS2) region of scuticociliates and
related taxa (Ciliophora, Oligohymenophorea) to infer their evolution and phylogeny.
Protist.
2008;
159
519-533
- 22
Schultz J, Maisel S, Gerlach D, Müller T, Wolf M.
A common core of secondary structure of the internal transcribed spacer 2 (ITS2) throughout
the Eukaryota.
RNA.
2005;
11
361-364
- 23
Tamura K, Dudley J, Nei M, Kumar S.
MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0.
Mol Biol Evol.
2007;
24
1596-1599
- 24
Meier R, Zhang G Y, Ali F.
The use of mean instead of smallest interspecific distances exaggerates the size of
the “barcoding gap” and leads to misidentification.
Syst Biol.
2008;
57
809-813
- 25
Ross H A, Murugan S, Li W L S.
Testing the reliability of genetic methods of species identification via simulation.
Syst Biol.
2008;
57
216-230
Prof. PhD Shilin Chen
Institute of Medicinal Plant Development
Chinese Academy of Medical Sciences
No. 151 Malianwa North Road, Haidian District
100193 Beijing
China
Phone: + 86 10 62 89 97 00
Fax: + 86 10 62 89 97 76
Email: slchen@implad.ac.cn