Role of Electrolytes and Glucose in the Insulin-Induced Electrochemical Effect in Sheep Pleura

Abstract

Aim: Insulin induces electrochemical alterations in sheep visceral and parietal pleura, an effect abolished by the Na⁺-channel blocker amiloride and the Na⁺-K⁺ pump inhibitor ouabain. The aim of this study was to further investigate the role of different electrolytes and glucose in these electrochemical changes.

Materials and methods: Sheep pleural specimens were mounted in Ussing chambers. Insulin (10⁻⁷M) was added mesothelially in Na⁺, K⁺, Ca²⁺-free, low H⁺ and glucose solutions. In other experiments, specimens were pretreated with K⁺ and Ca²⁺-free Krebs solutions. Trans-mesothelial Resistance was determined.

Results: Insulin did not increase Trans-mesothelial Resistance of visceral and parietal pleura in K⁺-free (p=0.008 and p=0.028 respectively), Ca²⁺-free (p=0.006 and p=0.012 respectively) and low glucose (p=0.009 and p=0.03 respectively) solutions. This effect was totally inhibited in Na⁺-free solutions or in specimens pretreated with Ca²⁺ -free Krebs solution and partially inhibited, when low H⁺ solutions were used (p=0.042 for visceral and p=0.045 for parietal).

Conclusion: Insulin-induced electrochemical changes in sheep pleura are mainly associated with alterations in Na⁺ and Ca²⁺ concentrations. Since amiloride and ouabain abolish these electrochemical changes, it may be suggested that insulin could influence the pleural fluid recycling, mainly via the Na⁺ transportation system, irrespective of the glucose content.

Key words

insulin - electrolytes - glucose - pleura - electrochemical

References

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