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DOI: 10.1055/s-0029-1243123
© Georg Thieme Verlag Stuttgart · New York
Quantitative Determination of Secoiridoid and γ-Pyrone Compounds in Gentiana lutea Cultured in vitro
Publication History
Received: February 5, 1999
Accepted: June 12, 1999
Publication Date:
24 November 2009 (online)
Abstract
The production of secondary metabolites was studied in shoots, roots, and hairy roots of Gentiana lutea obtained in vitro. In shoots, both secoiridoid and γ-pyrone compounds were detected in amounts similar to those found in aerial parts of plants collected from nature. The most abundant secoiridoid was gentiopicrin while mangiferin was the main compound among the γ-pyrones. The adventitious roots obtained in vitro showed a poor biosynthetic capacity. Upon infection with Agrobacterium rhizogenes, nine hairy root clones were established which differed in the amount of secondary metabolites.
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Dr. Nebojša Menković
Institute for Medicinal Plants Research
Tadeuša Košćuška 1
11000 Belgrade
Yugoslavia
Email: katarinasf@hotmail.com
Email: ksf@eunet.yu
Fax: +0038111182072