Planta Med 2010; 76(13): 1411-1418
DOI: 10.1055/s-0029-1240891
Pharmacology
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Chemical Composition and Evaluation of the Anti-Hypernociceptive Effect of the Essential Oil Extracted from the Leaves of Ugni myricoides on Inflammatory and Neuropathic Models of Pain in Mice

Nara L. M. Quintão1 , Gislaine F. da Silva1 , Carla S. Antonialli1 , Lilian W. Rocha1 , Valdir Cechinel Filho1 , José F. Cicció2
  • 1Programa de Mestrado em Ciências Farmacêuticas and Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), CCS, Universidade do Vale do Itajaí (UNIVALI), Itajaí-SC, Brazil
  • 2Centro de Investigaciones en Productos Naturales (CIPRONA) and Escuela de Química, Universidad de Costa Rica, Ciudad Universitaria Rodrigo Facio, San José, Costa Rica
Further Information

Publication History

received August 12, 2009 revised January 20, 2010

accepted January 24, 2010

Publication Date:
15 February 2010 (online)

Abstract

The study analyzes the chemical composition of the essential oil obtained from the leaves of Ugni myricoides (Kunth) O. Berg (U. myricoides EO). The composition of the essential oil was characterized by GC‐FID and GC‐MS analysis, showing at least six major constituents: α-pinene (52.1 %), 1,8-cineole (11.9 %), α-humulene (4.6 %), caryophyllene oxide + globulol (4.5 %), humulene epoxide II (4.2 %) and β-caryophyllene (2.9 %). It demonstrates for the first time the systemic anti-hypernociceptive properties of this orally administered oil in inflammatory and neuropathic models of hypernociception in mice. The effects of U. myricoides EO and its major constituent, α-pinene, were compared with those of indomethacin or gabapentin, drugs used clinically to treat inflammatory and neuropathic processes. Like indomethacin (5 or 10 mg/kg, p. o.), U. myricoides EO (5–50 mg/kg, p. o.) was able to significantly prevent mechanical hypernociception induced by carrageenan or complete Freund's adjuvant (CFA) in mice. These effects were observed for up to 48 h after i.pl. injection of flogistic agents. Repeated treatment with U. myricoides EO (5–25 mg/kg, p. o.), α-pinene (5–50 mg/kg, p. o.), or gabapentin (70 mg/kg, p. o.) also abolished the mechanical sensitization induced by CFA, or following the partial ligation of the sciatic nerve (PLSN). The present results indicate that U. myricoides EO produces marked anti-hypernociceptive effects in carrageenan and CFA mechanical sensitization models, and also inhibited neuropathic pain-like behavior after PLSN with efficacy similar to that observed for indomethacin or gabapentin. The relevant effects shown by U. myricoides EO are related, at least in part, to the presence of α-pinene and may be of potential interest for the management of inflammatory and neuropathic pain.

References

Prof. N. L. M. Quintão

Programa de Mestrado em Ciências Farmacêuticas
Universidade do Vale do Itajaí

Rua Uruguai, n°458

Bloco 27, CCS, sala 314

CEP 88302–202

Itajaí, SC

Brazil

Phone: + 55 47 33 41 79 32

Fax: + 55 47 33 41 76 52

Email: narafarmaco@yahoo.com.br

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