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Planta Med 2010; 76(10): 1026-1031
DOI: 10.1055/s-0029-1240850
Analytical Studies
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Comparative Analysis of Sambucus nigra and Sambucus australis Flowers: Development and Validation of an HPLC Method for Raw Material Quantification and Preliminary Stability Study

Marina Scopel1 , Lílian Auler Mentz2 , Amélia Teresinha Henriques1
  • 1Faculdade de Farmácia, Departamento de Farmacognosia, Universidade Federal do Rio Grande do Sul, Rio Grande do Sul, Brazil
  • 2Departamento de Botânica, Universidade Federal do Rio Grande do Sul, Rio Grande do Sul, Brazil
Weitere Informationen

Publikationsverlauf

received March 26, 2009 revised Dec. 30, 2009

accepted January 8, 2010

Publikationsdatum:
01. März 2010 (online)

    Lizenzen und Reprints

Abstract

This work was designed to develop a simple, effective, and reliable LC system to identify a chemical marker and compare Sambucus nigra L. and Sambucus australis Cham. et Schltdl. flower extracts (American and European elder). Rutin was the main constituent of both species. The developed method showed a linear response in the range of 10 to 45 µg · mL−1 for rutin and 1.75 to 3.25 µg · mL−1 for samples of the Sambucus species. Precision was determined and the relative standard deviations were 1.75 % for HSN and 1.28 % for HSA for intraday precision and 1.28 % and 1.51 % for inter-day precision, respectively, while accuracy was 97.9 % for HSN and 99.41 % for HSA. Quantification and detection limits as well as robustness were determined, presenting adequate results. The LC method showed an adequate performance for the separation of flavonoid glycosides in S. nigra and S. australis extracts, since the presence of interference had been previously evaluated. The analysis of thirty different samples of S. nigra and S. australis of different origins did not show significant variability among them. An accelerated stability study revealed a significant decrease in the first 30 days reaching 57 % in 90 days for S. australis samples and a total decrease of 25 % in 90 days for S. nigra samples, considering rutin as the chemical marker. These results will contribute to quality control analysis routines of these raw materials in pharmaceutical production facilities.

Key words

Sambucus nigra - Sambucus australis - Caprifoliaceae - stability study - flavonoids - HPLC

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Prof. Dr. Amélia T. Henriques

Departamento de Farmacognosia
Faculdade de Farmácia
Universidade Federal do Rio Grande do Sul

Av. Ipiranga, 2752, 505H

CEP 90610–000, Porto Alegre

Rio Grande do Sul

Brazil

Telefon: + 55 51 33 08 54 17

Fax: + 55 51 33 08 54 37

eMail: amélia@farmacia.ufrgs.br

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