Administration of Caffeic Acid Worsened Bone Mechanical Properties in Female Rats

Abstract

Natural phenolic acids, commonly present in plants that are normally consumed in the diet, have been reported to exert antiresorptive and/or bone formation increasing activity. The aim of the present study was to investigate the effects of ferulic, caffeic, p-coumaric, and chlorogenic acids on the skeletal system of normal, mature female rats. The phenolic acids (10 mg/kg p. o. daily for 4 weeks) were administered to 3-month-old female Wistar Cmd:(WI)WU rats. Bone mass, mineral and calcium content, macrometric and histomorphometric parameters, and mechanical properties were examined. Phenolic acids had differential effects on the rat skeletal system. Although none of them affected bone macrometric parameters, mass and mineralization, all of them increased the width of femoral trabeculae. Administration of caffeic acid worsened bone mechanical properties (decreasing ultimate load sustained by the femur in three-point bending test). In conclusion, high intake of caffeic acid may unfavorably affect the skeletal system.

Key words

ferulic acid - caffeic acid - p‐coumaric acid - chlorogenic acid - skeletal system - rats

References

• 1 Stalikas C D. Extraction, separation, and detection methods for phenolic acids and flavonoids.  J Sep Sci. 2007;  30 3268-3295
• 2 Kroon P A, Williamson G. Hydroxycinnamates in plants and food: current and future perspectives.  J Sci Food Agric. 1999;  79 355-361
• 3 Calabrese V, Mancuso C, Calvani M, Rizzarelli E, Butterfield D A, Giuffrida Stella A M. Nitric oxide in the central nervous system: neuroprotection versus neurotoxicity.  Nat Rev Neurosci. 2007;  8 766-775
• 4 Hsu C L, Yen G C. Phenolic compounds: evidence for inhibitory effects against obesity and their underlying molecular signaling mechanisms.  Mol Nutr Food Res. 2008;  52 53-61
• 5 Lai Y L, Yamaguchi M. Phytocomponent p-hydroxycinnamic acid stimulates bone formation and inhibits bone resorption in rat femoral tissues in vitro.  Mol Cell Biochem. 2006;  292 45-52
• 6 Lai Y L, Yamaguchi M. Phytocomponent p-hydroxycinnamic acid inhibits osteoclast-like cell formation in mouse bone marrow cultures.  Int J Mol Med. 2007;  19 123-128
• 7 Lai Y L, Yamaguchi M. Oral administration of phytocomponent p-hydroxycinnamic acid has anabolic effect on bone calcification in femoral tissues in rats in vivo.  J Health Sci. 2006;  52 308-312
• 8 Tang Q Y, Kukita T, Ushijima Y, Kukita A, Nagata K, Sandra F, Watanabe T, Toh K, Okuma Y, Kawasaki S, Rasubala L, Teramachi J, Miyamoto I, Wu Z, Iijima T. Regulation of osteoclastogenesis by Simon extracts composed of caffeic acid and related compounds: successful suppression of bone destruction accompanied with adjuvant-induced arthritis in rats.  Histochem Cell Biol. 2006;  125 215-225
• 9 Sassa S, Kikuchi T, Shinoda H, Suzuki S, Kudo H, Sakamoto S. Preventive effect of ferulic acid on bone loss in ovariectomized rats.  In Vivo. 2003;  17 277-280
• 10 Yamaguchi M, Uchiyama S, Lai Y L. Oral administration of phytocomponent p-hydroxycinnamic acid has a preventive effect on bone loss in streptozotocin-induced diabetic rats.  Int J Mol Med. 2007;  19 803-807
• 11 Yamaguchi M, Lai Y L, Uchiyama S, Nakagawa T. Oral administration of phytocomponent p-hydroxycinnamic acid prevents bone loss in ovariectomized rats.  Mol Cell Biochem. 2008;  311 31-36
• 12 Folwarczna J, Zych M, Burczyk J, Trzeciak H, Trzeciak H I. Effects of natural phenolic acids on the skeletal system of ovariectomized rats.  Planta Med. 2009;  DOI: 10.1055/s-0029-1185904
• 13 Folwarczna J, Pytlik M, Janiec W. Effects of doxycycline on development of changes in histomorphometric parameters of bones induced by bilateral ovariectomy in rats.  Pol J Pharmacol. 2003;  55 433-441
• 14 Folwarczna J, Śliwiński L, Cegieła U, Pytlik M, Kaczmarczyk-Sedlak I, Nowińska B, Janiec W, Trzeciak H I. Raloxifene similarly affects the skeletal system of male and ovariectomized female rats.  Pharmacol Rep. 2007;  59 349-358
• 15 Folwarczna J, Janiec W, Śliwiński L. Effects of heparin and low-molecular-weight heparins on bone mechanical properties in rats.  Thromb Haemost. 2004;  92 940-946
• 16 Pytlik M, Folwarczna J, Janiec W. Effects of doxycycline on mechanical properties of bones in rats with ovariectomy-induced osteopenia.  Calcif Tissue Int. 2004;  75 225-230
• 17 Kalu D N. The ovariectomized rat model of postmenopausal bone loss.  Bone Miner. 1991;  15 175-191
• 18 Hwang C S, Kwak H S, Lim H J, Lee S H, Kang Y S, Choe T B, Hur H G, Han K O. Isoflavone metabolites and their in vitro dual functions: they can act as an estrogenic agonist or antagonist depending on the estrogen concentration.  J Steroid Biochem Mol Biol. 2006;  101 246-253
• 19 Chang C J, Chiu J H, Tseng L M, Chang C H, Chien T M, Wu C W, Lui W Y. Modulation of HER2 expression by ferulic acid on human breast cancer MCF7 cells.  Eur J Clin Invest. 2006;  36 588-596
• 20 Stromeier S, Petereit F, Nahrstedt A. Phenolic esters from the rhizomes of Cimicifuga racemosa do not cause proliferation effects in MCF-7 cells.  Planta Med. 2005;  71 495-500
• 21 Zych M, Folwarczna J, Trzeciak H I. Natural phenolic acids may increase serum estradiol level in ovariectomized rats.  Acta Biochim Pol. 2009;  56 503-507
• 22 Clifford M N. Chlorogenic acids and other cinnamates – nature, occurrence and dietary burden.  J Sci Food Agric. 1999;  79 362-372
• 23 Higdon J V, Frei B. Coffee and health: a review of recent human research.  Crit Rev Food Sci Nutr. 2006;  46 101-123
• 24 Monteiro M, Farah A, Perrone D, Trugo L C, Donangelo C. Chlorogenic acid compounds from coffee are differentially absorbed and metabolized in humans.  J Nutr. 2007;  137 2196-2201