Planta Med 2009; 75 - PI1
DOI: 10.1055/s-0029-1234765

Chemical constituents and antibacterial activity against bacteria causing foot odor of three extraction methods from the pummelo peel oil

S Chuenpitayaton 1, R Temsiririrkkul 1, S Saraya 2, N Ruangwises 3, Y Wongkrajang 4, J Punsrirat 3
  • 1Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayudhaya Rd., Ratchatewi, Bangkok 10400, Thailand
  • 2Department of Microbiology, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayudhaya Rd., Ratchatewi, Bangkok 10400, Thailand
  • 3Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayudhaya Rd., Ratchatewi, Bangkok 10400, Thailand
  • 4Department of Physiology, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayudhaya Rd., Ratchatewi, Bangkok 10400, Thailand

The flavedo from fresh peels of Pummelo (Citrus maxima (Burm) Merr.) [1] were extracted by three different extraction methods; hydrodistillation [2], steam distillation and hexane extraction. The total yields from hydrodistillation, steam distillation and solvent extraction were 2.25, 1.83 and 1.47% w/w, respectively. Each oil samples were analyzed for chemical components by gas chromatograph and mass spectrometer (GC-MS). The highest content of monoterpene hydrocarbons i.e., limonene, phellandrene, α-pinene, were found in the extracts obtained from hydrodistillation (95.12%, 0.65%, and 0.61%, respectively). They were also investigated for their antimicrobial activities against bacterial causing foot odor, including Bacillus subtilis (ATCC 6633), Staphylococcus aureus (ATCC 6538), and Staphylococcus epidermidis (ATCC 12228) [3], using broth microdilution method [4]. The minimum inhibitory concentration (MIC) of the oil obtained from hydrodistillation with the most potency is 0.125, 0.125 and 0.03125% v/v for Bacillus subtilis, Staphylococcus aureus and Staphylococcus epidermidis, respectively.

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[3] Katsutoshi, A. et al. (2006) Can. J. Microbiol. 52:357–364.

[4] Ferraro, M.J. et al. (2000) Methods of dilution antimicrobial susceptibility test for bacteria that grow aerobically; approved standard-fifth. NCCLS. USA.